Bionex, Inc.

Bionix, Inc.

Gi Young Jang

Raehyuk Chang

Hee Joun Roh

The present invention relates to a kit for separating and purifying nucleic acids or a variety of biological materials and a system for automatically performing the operations of separating and purifying a variety of biological materials using the kit. The kit of the present invention comprises a container with a plurality of chambers formed in a row for containing buffers and solid materials suitable for separating the nucleic acids or biological materials from the biological samples, and a carriage including a fiat portion with a hole formed therethrough and a projection having an inside passage of which one end is closed and the other end is open and having a predetermined length such that the projection can be dipped into the buffers. A mounting means is formed on the flat portion of the carriage such that the carriage can be detachably mounted to a transport means for transferring the carriage to the respective chambers. Further, a system for automatically performing the separation and purification using the kit is also provided. According to the present invention, a predetermined number and a small amount of samples can be selectively processed in accordance with a user's needs. Therefore, the samples are quickly processed and prevented from being unnecessarily wasted, and thus, processing efficiency can be improved.

Kit for separating and purifying nucleic acids or various biological materials, and system for automatically performing separation or purification of biological materials using the same

The present invention relates to a method of manufacturing a kit for isolating and purifying nucleic acids, or various biological materials, a kit manufactured by the method, and an apparatus using the kit. The method of manufacturing a kit for isolating or purifying nucleic acids or biological materials from biological samples using solid materials comprises the steps of; fabricating a container having a plurality of chambers and/or column which is configured to contain the solid materials, buffers and/or enzymes suitable for isolation of the nucleic acids or biological materials; fabricating a cover including a protruded portion with a bore formed therethrough; filling each of the chambers or column with the solid materials, buffers and/or enzymes for isolation and purification of the biological materials or nucleic acids; sealing the container with a predetermined sealing material; and packaging the cover and the container, one by one, with a separate case. According to the present invention, the kit, which is simple, inexpensive and effective in a small or medium number of sample manipulations, can be manufactured. Thus, manual pipetting works by a person who has not fully trained for clinical or biological experiments can be avoided. Further, the disposable kit having the column in which the nucleic acids isolated from the biological samples have been already contained can be directly transferred to the conventional PCR cycler in order to perform the PCR amplification without changing any other buffers and tubes.

Method of manufacturing kit for isolation nucleic acids or biological materials

An automated centrifuge system. The centrifuge includes a rotor in which at least two swing buckets with at least two microplates mounted thereon are provided, a multi-joint robot with at least three joints which includes a gripper attached to a final arm thereof and a finger attached to the gripper, and a controller for controlling the predetermined procedures and operation of the robot. The centrifuge and multi-joint robot are disposed close to each other on a rigid bottom plate. A sensor for use in position detection is attached to the gripper or finger. At least one marker is attached at a predetermined position on a central frame of the rotor.

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Seoul, South Korea

Gi Young Jang