10x Genomics, Inc.

Population Genetics Technologies Ltd.

Agilent Technologies, Inc.

New England Biolabs, Inc.

Compass Genetics, LLC

Gi Mikawa

Sydney Brenner

Robert Osborne

Andrew Slatter

Esther Musgrave-Brown

James Casbon

Andreas Claas

Stephen C Macevicz

Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.

Methods for analyzing nucleic acids

Methods for analyzing nucleic acids from single cells

Described herein is a method for adding a counter sequence to the individual polynucleotide molecules of an initial nucleic acid sample. After addition of the counter sequence, the sample may be amplified and the number of initial target molecules in the sample can be estimated by counting the number of counter sequences associated with the amplified target molecules.

Method for attaching a counter sequence to a nucleic acid sample

Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide, also referred to as a 'reflex method'. In certain embodiments, the reflex method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or multiplex identifier). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided.

Compositions and methods for intramolecular nucleic acid rearrangement

Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide, also referred to as a 'reflex method'. In certain embodiments, the reflex method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or multiplex indentifier). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided.

The present invention provides methods and compositions for asymmetrically tagging a nucleic acid fragment using asymmetric adapters.

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Great Shelford, United Kingdom

Gi Mikawa