Bioarray Solutions Ltd.

New York Blood Center, Inc.

Bioinventors & Entrepreneurs Network, LLC

Bioinventors & Entrepeneurs Network LLC

Seul, Michael

Reid, Marion E.

Ghazala Hashmi

Michael Seul

Joachim Messing

Michael D Pierce

Marion E Reid

Bhubaneswar Mishra

Alice Xiang Li

Vera Cherepinsky

Richard Klemm

Michael Pierce

The invention provides methods and processes for the identification of polymorphisms at one ore more designated sites, without interference from non-designated sites located within proximity of such designated sites. Probes are provided capable of interrogation of such designated sites in order to determine the composition of each such designated site. By the methods of this invention, one ore more mutations within the CFTR gene and the HLA gene complex can be identified.

Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection

A process of identifying a plurality of biological samples having particular desired attributes by testing pooled samples and selecting, for intended uses such as transfusion, or for subsequent analysis that is thereby enriched for such samples, pooled samples which have, or may have, said desired attributes. The preferred number of samples per pool 'd' is determined by selecting an integer value as d which produces the maximum or a value near the maximum of the product of: d times the expected number of unambiguous sample pools, where a sample pool is unambiguous if all of the samples have the desired attributes, and is otherwise ambiguous if at least one sample has the desired attributes. The value selected as d can be greater than the maximum product above, so as to enlarge the total number of samples assayed in determining the desired attributes.

Attribute sieving and profiling with sample enrichment by optimized pooling

Display screen or portion thereof with a graphical user interface

Disclosed is an analysis method useful in multiplexed hybridization-mediated analysis of polymorphisms, i.e., wherein a labeled nucleic acid of interest ('target') interacts with two or more pairs of immobilized degenerate capture probes. In one embodiment, one member of each pair has a sequence that is complementary to the normal ('wild-type') sequence in a designated location of the target, while the other member of each pair has a sequence that is complementary to an anticipated variant (&#x201c;mutant&#x201d; or &#x201c;polymorph&#x201d;) sequence in that location of the target. These methods permit selection of two or more probe pairs such that, for each pair of probes interacting with a given target strand, interaction of the target with a preferred member of the probe pair is optimized. Also interpreting results obtained by multiplexed hybridization of the target to two or more pairs of probes under conditions permitting competitive hybridization is disclosed.

Method of probe design for nucleic acid analysis by multiplexed hybridization

Described are methods of assay design and assay image correction, useful for multiplexed genetic screening for mutations and polymorphisms, including CF-related mutants and polymorphs, using an array of probe pairs (in one aspect, where one member is complementary to a particular mutant or polymorphic allele and the other member is complementary to a corresponding wild type allele), with probes bound to encoded particles (e.g., beads) wherein the encoding allows identification of the attached probe. The methods relate to avoiding cross-hybridization by selection of probes and amplicons, as well as separation of reactions of certain probes and amplicons where a homology threshold is exceeded. Methods of correcting a fluorescent image using a background map, where the particles also contain an optical encoding system, are also disclosed.

Correcting an assay image of an array of signals generated from a multiplexed hybridization-mediated assay

This invention provides compositions and methods for genetic testing of an organism and for correlating the results of the genetic testing with a unique marker that unambiguously identifies the organism. The markers may be internal markers, such as for example single nucleotide polymorphisms (SNPs), short tandem repeats (STRs), or other sites within a genomic locus. Alternatively, the markers may be external, such that they are separately added to the genetic sample before testing.

Analyzing blood type with identification of patient by genotyping

Disclosed are a method and an algorithm for genetic cross-matching based on the comparison of recipient and donor genotypes&#x2014;and the underlying combinations of alleles and haplotypes. The method of the invention, rather than focusing on phenotype prediction, instead relies on a comparison of genetic variants identified in the recipient and available donors, whose information preferably will be compiled in a widely available donor registry, to maximize molecular compatibility. The genotypes can be matched based on the weighted clinical significance of a genotypic difference between donor and recipient, such that certain mismatches are more acceptable than others.

Primers for exons of variants of RHCE and RHD genes

Differentiating homozygous, heterozygous and wild-type alleles using a multiplexed hybridization-mediated assay

Method of nucleic acid typing for selecting registered donors for cross-matching to transfusion recipients

Genetic analysis and authentication

Real-time monitoring of nucleic acid target-identifying signals

Determination of the number of tandem repeat nucleotides using encoded probe-displaying beads

Growing community of inventors

Holmdel, NJ, United States of America

Ghazala Hashmi