Abm Technologies, LLC

Universidad Autónoma De Madrid

Germán Bou Arévalo

Rebeca Santiso Brandariz

Maria Tamayo Novas

Jaime Gosálvez Berenguer

José Luis Fernández García

Jaime Gosalvez Berenguer

Jose Luis Fernandez Garcia

Fátima María Otero Fariña

Fatima Maria Otero Farina

María Tamayo Novás

A method of rapidly evaluating the susceptibility of a strain of bacteria to a cell wall synthesis inhibiting antibiotic based on an assessment of cell enlargement in response to doses of the cell wall synthesis inhibiting antibiotic which are correlated to breakpoints of bacterial susceptibility.

Method for the rapid determination of susceptibility or resistance of bacteria to antibiotics

The present invention relates to a method for the rapid evaluation of bacterial susceptibility or non-susceptibility of bacteria to antibiotics that inhibit protein synthesis. The rationale is to identify bacterial responses that depend or are influenced by protein synthesis. If this response is prevented or reduced by the antibiotic that inhibits the protein synthesis, the bacteria are susceptible to this antibiotic. Otherwise, if the response keeps similar despite the incubation with the antibiotic, the bacteria are not susceptible or resistant to this antibiotic. These responses could be determined at the DNA lev-el, cell wall level, morphological level or any other experimental approach, including metabolic, bio-chemical, physiological or genetic processes.

Procedure for the rapid determination of bacterial susceptibility to antibiotics that inhibit protein synthesis

The present invention relates to a method for evaluating the integrity of the cell wall of the bacteria present in a culture in the presence of an antibiotic acting on the bacterial cell wall which, from a practical point of view, allows quickly determining if a bacterium is sensitive or resistant to an antibiotic acting on the bacterial cell wall. Likewise, the present invention also relates to a lysis solution applicable in the preceding method, specifically affecting bacteria having the cell wall damaged by the action of an antibiotic acting on the bacterial cell wall, which allows distinguishing bacteria sensitive to said antibiotic from those resistant to said antibiotic.

Method for evaluating bacterial cell wall integrity

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Culleredo, Spain

Germán Bou Arévalo