Bruker Ag

Bruker Biospin Ag

Gerhard Wider

Konstantin Pervushin

Roland Riek

Kurt Wuthrich

Kurt Wuethrich

Sebastian Hiller

Polarization transfer by cross-correlated relaxation in solution NMR with very large molecules

A method for obtaining a nuclear magnetic resonance (NMR) correlation spectrum of heteronuclear spin systems, in particular comprising large molecules, especially biological macromolecules in solution, the spin system being subjected to a homogeneous magnetic field B.sub.0, being irradiated by a sequence of radio frequency (rf) pulses, is characterized in that the spin system comprises at least two kinds of spin 1/2 nuclei I and S being coupled to each other, whereby the sequence of rf pulses is chosen such that line broadening in the observed spectrum due to transverse relaxation (T.sub.2) is significantly reduced because of cross correlation between dipole--dipole (DD) coupling of the spins and chemical shift anisotropy (CSA), giving rise to different relaxation rates of the individual multiplet components of the spin system and chosen such that the relaxation effects of the two different mechanisms cancel each other out to a large degree. Thus, even very large biological macromolecules can be measured.

Transverse relaxation-optimized spectroscopy (=TROSY)

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Herrliberg, Switzerland

Gerhard Wider