Becton Dickinson and Company Limited

Tecan Trading Ag

Ffe Service Gmbh

Siemens Aktiengesellschaft

Gerhard Weber

Peter Weber

Christoph Eckerskorn

Hans-Jörg Grill

David Yost

Wolfgang Sitter

Christoph Weber

Thomas Thanner

Mikkel Nissum

The present invention is related to a free-flow electrophoresis method for separating at least one analyte of interest from a mixture of analytes, wherein the method uses a separation medium comprising two or more individual separation media, wherein the two or more individual separation media differ in their pH value, and wherein each of the two or more individual separation media comprise at least one anion of at least one acid and at least one cation of at least one base, wherein the at least one acid is the same in each of the two or more individual separation media and the at least one base is the same in each of the two or more individual separation media.

Free-flow electrophoresis method for separating analytes

The invention relates to a method and apparatus for carrier-free deflection electrophoresis, in which a separating media and a sample to be examined flow through a separating chamber between a pair of electrodes in a series of reversing bulk fluid flow along the direction of the electrodes, thereby separating the sample into zones which are to be collected into fractions for analysis or further processing. Among other things, the apparatus and method enable high-resolution separation of particles that can be performed in miniaturized chambers in electrophoresis modes including isoelectric focusing, zone electrophoresis, and isotachophoresis.

Methods and apparatus for carrier-free deflection electrophoresis

An electrophoresis method is provided involving a parallel and simultaneous multiple process, using multiple separation sub-spaces within a separation chamber.

Method for electrophoresis involving parallel and simultaneous separation

A free flow electrophoresis method for separating sample substances into their analytes. This method comprises multiple steps: a first step for the crude fractionation of the sample substance and at least one second step in which the crudely fractionated sample substance is finely fractionated. These steps can be performed in a parallel simultaneous method, in a serial method or in a combination of these two methods.

Carrierless electrophoresis process and electrophoresis device for carrying out this process

An electrophoresis device having a separation chamber that is provided with at least one sample inlet on the inlet side and outlets () for the electrophoretically treated sample species on the outlet side. The separation chamber is divided into two chamber parts () by at least one separation element () which is selectively permeable for specific sample species and has a continuous inner space extending longitudinally, especially a hollow fiber, from the inlet to the outlet side. Electrodes () are disposed parallel to the separation element on both sides of the separation chamber.

Electrophoresis device, electrophoresis method using an electrophoresis device and use of the electrophoresis device

The invention relates to a medium for analytic and preparative electrophoresis which includes a content of acids and bases of different pKa values. The medium contains at least two acids, where the difference in pKa values (&#x394;pKa) of adjacent acids are no greater than 1.0, preferably in the range from 0.8 to 0.5. The medium also contains at least two bases, whose pKa values range from approximately 1.5 to 11, and the difference of the pKa values (&#x394;pKa) of adjacent bases is no greater than 1.0, preferably in the range from 0.8 to 0.5.

Medium for analytic and preparative electrophoresis

A free-flow electrophoresis (FFE) apparatus and separation medium for separating particles therefore, includes a separation chamber with a dosage pump for conveying medium, electrodes for applying an electric field in the medium and sample injection points for adding a mixture of particles to be separated and fractionation points for removing the particles. Definable charge labels couple with the particles to form charge-modified particles which display with a different migratory behavior in FFE The apparatus includes focusing buffers between the separation medium and at least one electrode which has much higher electrical conductivity than the separation medium.

Apparatus and separation media for separating particles in free-flow electrophoresis

A method for separating particles in free-flow electrophoresis FFE includes conveying a separation medium using a dosage pump into a separation chamber; releasing the separation medium via outlets; applying an electrical field in the separation medium; coupling a particle to be separated with selectively defined charge labels for generating charge-modified particles which due to their selectively modified net surface charge display a different migratory behavior in FFE than non-charge-modified particles; adding a mixture of particles to be separated to the separation medium in the separation chamber by way of sample injection points; removing the particles separated using the separation medium by way of fractionation points; and collecting the fractions with the separated particles. Focusing buffers are created using a buffer medium between the separation medium and at least one electrode, whereby this buffer medium is much more electrically conductive than the separation medium and is fed into these focusing buffers.

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Kirchheim, Germany

Gerhard Weber