Harvard College

The General Hospital Corporation

Dana-farber-cancer Institute Inc.

Cellmig Biolabs Inc.

Children's Medical Center Corporation

Centre Hospitalier Universitaire Vaudois

Gerhard Wagner

Tingfang Yi

Michael Chorev

Bertal Huseyin Aktas

Haribabu Arthanari

Joy Luz Nishikawa

Sebastian Hiller

Jose Alberto Halperin

Judy Lieberman

Sara Jean Buhrlage

Ellis L Reinherz

Anders M Naar

José A Halperin

Huseyin Aktas

Kazuhisa Nishizawa

Jing Li

Elizabeth M O'Day

Nathan John Moerke

Andras Pal Boeszoermenyi

Christian Freund

Dominique Sanglard

Nathan Moerke

Ricard A Rodriguez-Mias

Mahmoud Nasr

Anders Näär

Patrick R Hagner

This invention provides for a network of cell-derived microfilaments. Also provided are methods of producing a network of microfilaments via culturing cells in a matrix support and cell culture medium wherein the cells proliferate and form aggregated cell masses, which produce microfilaments external to and surrounding the cell masses, and wherein the extracellular microfilaments connect and form a continuous extracellular microfilament network, and methods for treating a medical condition as well as facilitating wound repair and tissue regeneration comprising applying the microfilament network to an area in need of treatment.

Methods for generation of cell-derived microfilament network

This invention provides for methods and compositions for generation of cytocapsulae and cytocapsular tubes in a 3D matrix.

Methods for generation of cytocapsulae and cytocapsular tubes

Provided herein are compounds (e.g., compounds of Formulae (I), (II), and (III)) which are anti-fungal agents and can be used in the treatment of diseases, including infectious diseases. The invention provides methods of treating diseases in a subject (e.g., infectious diseases such as fungal infections), and methods of killing or inhibiting the growth of fungi in or on a subject or biological sample. The compounds may be used in subjects, in clinical settings, or in agricultural settings. 

Antifungal compounds and uses thereof

Described herein are methods and compositions relating to nanodiscs, e.g., phospholipid bilayers with a proteinaceous belt or border. Further provided herein are loopable membrane scaffold proteins, e.g., for forming nanodiscs.

Methods and compositions relating to covalently circularized nanodiscs

Described are methods and apparatus, referred to as 'temperature-lock,' which can control and stabilize the sample temperature in an NMR spectrometer, in some instances with a precision and an accuracy of below about 0.1 K. In conventional setups, sample heating caused by experiments with high-power radio frequency pulses is not readily detected and is corrected by a cumbersome manual procedure. In contrast, the temperature-lock disclosed herein automatically maintains the sample at the same reference temperature over the course of different NMR experiments. The temperature-lock can work by continuous or non-continuous measurement of the resonance frequency of a suitable temperature-lock nucleus and simultaneous adaptation of a temperature control signal to stabilize the sample at a reference temperature value. Inter-scan periods with variable length can be used to maintain the sample at thermal equilibrium over the full length of an experiment.

Methods and apparatus for sample temperature control in NMR spectrometers

Methods for inhibiting translation, treating a cellular proliferative disorder, and inhibiting proliferation of cells using the compounds disclosed herein are provided.

Methods and compounds for the inhibition of cellular proliferation

Methods that enable one to specifically measure the metabolic product of a particular molecule in relatively few cells, e.g. primary cells, are described. The methods involve optionally preloading cells with labeled substrate (e.g. labeled byC,N, orP). The methods allow for easy identification of metabolites that are differentially generated in cells of different phenotypes. The new methods for unbiased multi-dimensional NMR screening and rapid and efficient analysis of the NMR screening identify differentially expressed metabolites in different cell or tissue types. Analysis of the differentially expressed metabolites can present unique druggable targets to which small molecule therapeutics can be designed.

NMR-based metabolite screening platform

Compositions and methods for inhibiting translation are provided. Compositions, methods and kits for treating (1) cellular proliferative disorders, (2) non-proliferative, degenerative disorders, (3) viral infections, (4) disorders associated with viral infections, and/or (5) non-proliferative metabolic disorders such as type II diabetes where inhibition of translation initiation is beneficial using the compounds disclosed herein.

Compounds for the inhibition of cellular proliferation

The present invention relates to the identification of molecular mechanisms associated with multidrug resistance (MDR) in fungal infections. More specifically, fungi harbor a nuclear receptor-like pathway controlling MDR, which represents a novel therapeutic target for the treatment of MDR in pathogenic fungi such as

High throughput screens for small-molecule inhibitors of the nuclear receptor-like pathway regulating multidrug resistance in fungi

A composition and method for inhibiting proliferation of a tumor cell compared to a non-tumor cell. Also described are methods of screening for a composition that inhibits cap-dependent translation compared to cap-independent translation of proteins. 

Regulation of protein synthesis

A human CD2 cytoplasmic tail binding protein, CD2BP2, is described, as well as the nucleic acids encoding the protein. Also described are expression vectors and recombinant host cells comprising nucleic acids encoding the CD2BP2 protein, and methods of use for the CD2BP2 protein and nucleic acids encoding the CD2BP2 protein.

Growing community of inventors

Chestnut Hill, MA, United States of America

Gerhard Wagner