Merck Patent Gesellschaft MIT Beschrankter Haftung

Gerhard Pfleiderer

Norbert Hennrich

Siegfried Neumann

Hermann Lang

Hans-Dieter Orth

Hans Dieter Orth

Hans Pauly

Evangelista Jockers-Wretou

A process for the quantitative immunological determination of an enzyme in a liquid, comprises incubating anti-enzyme antibodies which are adsorbed on a water-insoluble carrier, with the sample solution containing the enzyme, whereby the enzyme bonds to the antibodies but retains a reproducible proportion of its activity in its complex with the antibodies; removing the sample solution; washing the antibody carrier, which is charged with enzyme; and determining the activity of the enzyme bonded to the antibodies.

Method and agent for the immunological determination of enzymes

Isoenzyme patterns of an enzyme in biological fluids, extracts, and/or excretions are quantitatively determined by precipitating at least 90% of an isoenzyme antigen with a corresponding heterologous antiserum and comparing the remaining enzyme activity with the total activity with the use of a conventional agent for enzyme activity determination.

Analysis of isoenzyme patterns

This invention relates to a process, to a diagnostic agent employed therein and to the use thereof, for the organ-specific and/or disease-specific diagnosis of the isozyme pattern of an enzyme occurring in multiple molecular configurations in a sample of human body fluids, tissue extracts or excretions, wherein total enzyme activity is quantitatively measured employing a conventional agent for the determination of enzyme activity, which comprises selectively forming an isozyme-antibody complex of a diagnostically relevant isozyme of the isozyme pattern of the enzyme present in the sample by employing a homologous antisera against the same human isozyme in a form free from the immunological activity of other isozymes of the same enzyme, separating the thus-formed isozyme-antibody complex substantially quantitatively, and measuring residual enzyme activity of the enzyme present in the sample.

Immunological isozyme determination method

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Witten, Germany

Gerhard Pfleiderer