Fidicula Gmbh

Fritz Biochem Gesellschaft Für Bioanalytik Mbh

Harald Lossau

Friz Biochem Gesellschaft Fur Bioanalytik Mbh

Gerhard Hartwich

Adam Heller

Peter Frischmann

Elisenda Ferrer

Norbert Persike

Philip Johnsen

Ute Haker

The invention relates to compounds of formula (I), wherein Z represents a hydrocarbon with 2 to 28 C atoms, wherein Z can also comprise elements N, O, P, S, Si and halogen as heteroatoms, Rand Rare identical or different sulfur protecting groups, wherein both S atoms can also form a disulfide bridge and in said case Rand Rare not present; protecting group Yrepresents protecting group NH, protecting group NR, protecting group O, CONH protecting group, protecting group OOC, protecting group S&#x2014;S, &#x2014;CH(protecting group O)or &#x2014;CR(protecting group O)or protecting group S; Yrepresents &#x2014;OH, &#x2014;NH, &#x2014;NHR, &#x2014;NRR, &#x2014;COOH, &#x2014;COCI, &#x2014;COOCO&#x2014;R, &#x2014;CONH, &#x2014;CONHR, &#x2014;COOR, &#x2014;SOH, &#x2014;SOI, &#x2014;SH, &#x2014;S&#x2014;SR, &#x2014;CHO, &#x2014;COR, &#x2014;CHO, halogen, &#x2014;N, &#x2014;NH&#x2014;NH, &#x2014;NCO, &#x2014;NCS, wherein Rrepresents alkyl, heteroalkyl, aryl, cycloalkyl or a protecting group, wherein Rcan be identical or different in groups Yand Y, Ris s protecting group, wherein Rcan be identical or different in groups Yand Y, and wherein Rand Rcan be identical or different, Rrepresents alkyl, aryl or cycloalkyl, wherein Rcan be identical or different in groups Yand Yand Rrepresents alkyl, heteroalkyl, aryl or cycloalkyl, wherein Rcan be identical or different in groups Yand Y, wherein Ycan also represent a group of formula (II) or formula (III), wherein Xrepresents halogen or a substituted amine, Xrepresents an alkyl, alkoxy, aryloxy radical or a cyano derivative of an alkyl, alkoxy, aryloxy radical, Xrepresents halogen, an amino function or oxygen and Xrepresents an alkyl, alkoxy, aryloxy radical or Xequals H if X=oxygen. 

Multifunctional reagent for the synthesis of thiol modified oligomers

The invention relates to a method for the electrochemical detection of sequence-specific nucleic acid oligomer hybridization events. To this end single DNA/RNA/PNA oligomer strands which at one end are covalently joined to a support surface and at the other, free end, covalently linked to a redox pair, are used as hybridization matrix (probe). As a result of treatment with the oligonucleotide solution (target) to be examined, the electric communication between the conductive support surface and the redox pair bridged by the single-strand oligonucleotide, which communication initially is either absent or very weak, is modified. In case of hybridization, the electric communication between the support surface and the redox pair, which is now bridged by a hybridized double-strand oligonucleotide, is increased. This permits the detection of a hybridization event by electrochemical methods such as cyclic voltametry, amperometry or conductivity measurement.

Method of the electrochemical detection of nucleic acid oligomer hybrids

An apparatus for detecting organic molecules, especially biomolecules and polymers, in a probe substance, comprises a test system () comprising an array of test sites (T) to which the probe substance can be supplied, each test site having specific probe molecules (), an illumination system () for optically illuminating a sub-array of test sites (T), and a detection system () for identifying those test sites (T) whose probe molecules () interact with the organic molecules () to be detected, the illumination system () comprising an array of independently addressable illumination sources (B) arranged such that to each test site (T) of the sub-array is assigned at least one illumination source (B) that illuminates substantially only it.

Device and method for detecting organic molecules in a test substance

Growing community of inventors

Munich, Germany

Gerhard Hartwich