Phadia Ab

Johnson & Johnson Gmbh

Amic Ab

Gerd Rundström

Ib Mendel-Hartvig

Paul Christopher

Per Matsson

Per Ove Öhman

Christer Pettersson

Rune Björkman

There is provided a method for the analysis of circulating antibodies comprising the steps:

Method for the analysis of circulating antibodies

A two step lateral flow assay method for identifying IgE antibodies in a sample comprises applying a sample to a sample port of a device, wherein the device is adapted to deliver the sample to a lateral flow matrix having a plurality of IgE antigen species immobilized at respective positions at a first location; allowing the sample to travel along the lateral flow matrix through the immobilized plurality of IgE antigen species to a second location downstream of the first location; and, after a predetermined period of time, applying liquid buffer to the lateral flow matrix to mobilize labeled reagent which is adapted to bind anti-IgE antibody and which is dried on the lateral flow matrix at a location upstream of the sample port delivery of the sample to the lateral flow matrix, and allowing labeled reagent mobilized by the liquid buffer to travel along the lateral flow matrix through the immobilized plurality of IgE antigen species and bind with any IgE antibody bound to the immobilized IgE antigen species, and to travel to a second location downstream of the first location where the mobilized labeled reagent causes a visible change to occur at the second location.

Two step lateral flow assay methods and devices

An analysis device having at least one sample addition zone, at least one sink, and at least one flow path connecting the at least one sample addition zone and the at least one sink. The at least one flow path includes projections substantially vertical to the surface of the substrate and having a height (H), diameter (D) and reciprocal spacing (t, t) such that lateral capillary flow of a liquid sample is achieved. The device includes at least two reaction zones in series, wherein each reaction zone is adapted to facilitating measurement of a response originating from one and the same analyte, and wherein the reaction zones are positioned to allow calculation of the concentration of at least one analyte. Advantages include that a more accurate value can be calculated, variations are reduced, and an estimation of the uncertainty of the response can be calculated.

Assay device comprising serial reaction zones

Lateral flow assay devices and methods for detecting an analyte in a sample which comprises a plurality of nonspecific binding pair members are adapted for two step determinations. In one embodiment, a method for identifying IgE antibodies in a sample comprises applying a sample to a sample port of a device, wherein the device is adapted to deliver the sample to a lateral flow matrix having a plurality of IgE antigen species immobilized at respective positions at a first location, allowing the sample to travel along the lateral flow matrix through the immobilized plurality of IgE antigen species to a second location downstream of the first location and allowing sample to dissolve a water-soluble mark, applying liquid buffer to the lateral flow matrix to mobilize labeled reagent which is adapted to bind anti-IgE antibody and which is dried on the lateral flow matrix at a location upstream of the sample delivery location, and allowing labeled reagent to bind any IgE antibody bound to the immobilized IgE antigen species therein.

Growing community of inventors

Uppsala, Sweden

Gerd Rundström