Qiagen Gmbh

Gerd Grosshauser

Ralf Himmelreich

Thomas Rothmann

Andy Wende

Christian Korfhage

Markus Jeziorski

Rainer Dahlke

Hans Robert Attig

Michael Eberhard

Josef Drexler

Eva Holzer

Hans Attig

The present invention pertains inter alia to a method for analyzing a sample comprising biomolecules, which comprises the following steps: Furthermore, specifically adapted processing cartridges and systems are provided.

Sample processing method and sample processing cartridge

A method for determining whether a lysate contains sufficient biological sample material for a nucleic acid amplification reaction that includes preparing the lysate in the presence of at least one compound that inhibits the amplification reaction if insufficient biological sample material was present during preparation of the lysate, but does not inhibit the amplification reaction if sufficient biological sample material was present during preparation of the lysate, subjecting the lysate to the amplification reaction, and analyzing a result of the amplification reaction. Due to the presence of the compound in the amplification reaction, no amplification signal is obtained if insufficient biological sample material was present during preparation of the lysate but an amplification signal is obtained if sufficient biological sample material was present during preparation of the lysate. The invention allows for reliable identification of false negatives that occur because insufficient sample material was subjected to the amplification assay.

Control for diagnostic assay

The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.

Helicase dependent isothermal amplification using nicking enzymes

Growing community of inventors

Pulheim, Germany

Gerd Grosshauser