Basf Aktiengesellschaft

Rhein Biotech Gesellschaft Fur Neue Biotechnologische Prozesse Und Produkte Mbh

Artes Biotechnology Gmbh

Rhein Biotech Gesellschaft

Gellissen, Gerd, Prof. Dr.

Gerd Gellissen

Claus Bollschweiler

Jurgen Schweden

Alexander Wm Strasser

Ralph H Pries

Ulrike Weydemann

Michael Piontek

Jens Klabunde

Cornelis Hollenberg

Adelheid Degelmann

Gerhard Braus

Sven Krappmann

Claudio De Virgilio

A heterologous protein is produced by a method in which yeast-type host cells are prepared, which, in addition to the at least one endogenous homologous DNA sequence coding for a first calnexin, includes at least one recombinant DNA sequence coding for the protein and at least one additional recombinant DNA sequence coding for at least one second calnexin. The protein in the host cell represents a heterologous secretable protein. The host cells are induced to express the heterologous protein via the at least one sequence coding for the protein and to overexpress calnexins by the at least one additional sequence coding for the at least one second calnexin. The host cells are then induced to secrete the heterologous protein. The secreted heterologous protein is then separated off.

Method for production of a heterologous protein using yeast-type host cells

The invention relates to a nucleic acid molecule, comprising a nucleic acid which codes for a polypeptide with chorismate mutase activity and derivatives thereof, whereby the derivatives have at least 10% of the chorismate mutase activity of the chorismate mutase, according to the identification number of the SEQ ID NO:2. The invention further relates to vectors containing nucleic acid molecules, to host cells comprising nucleic acid molecules and their use in methods for producing polypetides with chorismate mutase activity. The invention also relates to the polypeptides with chorismate mutase activity and antibodies which specifically recognize the same. In addition, the invention relates to methods for producing auxotrophic yeast strains using the nucleic acid molecules and to the preparation of the yeast strains. The invention also relates to the use of yeast strains together with the inventive vectors in methods for the recombinant expression of heterologous genes which allow the transformants to be easily selected by functional complementation.

Nucleic acid molecule, comprising a nucleic acid coding for a polypeptide with chorismate mutase activity

A process for the recombinant production of proteins in yeast comprises transforming the yeast with an expression cassette which comprises the following structural elements encoded:

Recombinant production of proteins in yeast

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Wülfrath, Germany

Gerd Gellissen