Dsm N.v.

Gist-brocades N.v.

Gist-brocades B.v.

Gerardus Cornelius Selten

Bart Willem Swinkels

Robertus Franciscus Van Gorcom

Annemarie Eveline Veenstra

Willem Van Hartingsveldt

Petrus Andreas Van Paridon

Rudolf Gm Luiten

Robert Fm Van Gorcom

The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.

Transformed fungal strains free of selection marker

Selection marker gene free recombinant strains: a method for obtaining

Selection marker gene free recombinant strains: method for obtaining

A nucleotide sequence encoding phytase has been isolated and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce phytase on an industrial scale. The phytase produced via the present invention may be used in a variety of processes requiring the conversion of phytate to inositol and inorganic phosphate.

Cloning and expression of phytase from aspergillus

Growing community of inventors

Berkel en Rodenrijs, Netherlands

Gerardus Cornelius Selten