Illumina Cambridge Limited

Illumina Corporation

Solexa, Inc.

Gerardo Turcatti

Anthony Romieu

Colin Lloyd Barnes

Roberto Rigatti

Geoffrey Paul Smith

John Milton

Xiaolin Wu

Tobias William Barr Ost

David James Earnshaw

Graham John Worsley

Xiaohai Liu

Eric H Kawashima

Pascal Mayer

Milan Fedurco

Celine Adessi

Isabelle Rasolonjatovo

Jean-Jacques Mermod

Isabelle Marie Julia Rasolonjatovo

Jonathan Mark Boutell

Niall Anthony Gormley

Jean-Jaques Mermod

Liu Xiaohai

Céline Adessi

David James Solexa Limited Earnshaw

Roberto Solexa Limited Rigatti

Tobias William Barr Solexa Limited Ost

Xiaohai Solexa Limited Liu

Isabelle Marie J Solexa Limited Rasolonjatovo

Geoffrey Paul Solexa Limited Smith

Graham John Solexa Limited Worsley

Colin Solexa Limited Barnes

John Solexa Limited Milton

Xiaolin Solexa Limited Wu

Eric Kawashima

Isabelle Marie J Rasolonjatovo

The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5' end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Preparation of templates for nucleic acid sequencing

Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5' end an oligonucleotide sequence Y and at the 3&#x2032; end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5&#x2032; end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5&#x2032; end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5&#x2032; ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.

Methods of nucleic acid amplification and sequencing

The invention relates to an additive which can be added to buffers used in nucleotide detection processes and improved methods of nucleic acid sequencing using this additive. In particular the invention relates to use of the additive to improve the efficiency of fluorescence-based multiple cycle nucleic acid sequencing reactions.

Method of nucleotide detection

The invention relates to an additive which can be added to buffers used in nucleotide detection processes and improved methods of nucleic acid sequencing using the additive. In particular the invention relates to use of the additive to improve the efficiency of fluorescence-based multiple cycle nucleic acid sequencing reactions.

The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.

Preparation of nucleic acid templates for solid phase amplification

This invention provides a new method of obtaining a high density, reproducible and uniform coverage of a solid surface, compounds suitable for such a method and methods of preparing such compounds. This invention further relates to methods of the chemical modification (carboxylation) of solid surfaces and their subsequent use for the attachment of amine-containing molecules including DNA, proteins and other polymers.

Conversion of amine- to carboxyl groups on solid surfaces

Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5' end an oligonucleotide sequence Y and at the 3&#x2032; end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5&#x2032; end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5&#x2032; end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5&#x2032; ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.

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Geneva, Switzerland

Gerardo Turcatti