Dsm N.v.

Gist-Brocades N.v.

Gerard Wagemaker

Yvonne Johanna Vos

Yvonne J Vos

Robert Willem Van Leen

Robert W Van Leen

Lambertus Christiaan Johannes Dorssers

Lambertus C Dorssers

Maria Ludovicus Nicolaas Persoon

Robert William Van Leen

Lambertus Christian Johannes Dorssers

Maria L Persoon

Expression and purification of human interleukin-3 and muteins thereof

The invention relates to nucleotide sequences encoding human interleukin-3 (hIL-3) as well as recombinant DNAs, expression cassettes, transformed host cells, and recombinant expression methods comprising such sequences. Additionally, the invention describes proteins having hIL-3 activity, as purified, recombinantly produced, or fusion protein forms of hIL-3, as well as methods of using such proteins to produce antibodies capable of immunospecific reaction with hIL-3.

Molecular cloning and expression of the Pro8 isoform of human IL-3

Methods are provided for improved production of hIL-3 either in glycosylated form from mammalian and yeast cells or in unglycosylated form from prokaryotes. Recombinantly produced human IL-3 is purified in a series of steps, initially employing hydrophobic interaction, followed by ion exchange chromatography and gel filtration.

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Den Haag, Netherlands

Gerard Wagemaker