Cis Bio International

Compagnie Oris Industrie S.a.

Compagnie Oris Industrie

Compagnie Oris Industries

Gerard Mathis

Jean-Marie Lehn

Christophe Dumont

Etienne J Jolu

Dominique Pouyat

Robert Deschenaux

Etienne Jolu

Beatrice Alpha

Michel Mabile

Eric Trinquet

Thierry Davin

Etienne Jean-Pierre Jolu

Daniel Aspe

Herve Autiero

Christine O Roth

Muriel Foyentin

Dominique Nuti

The invention relates to a process for reducing the fluorescence quenching caused by the measuring medium, in a fluorescence assay for an analyte using at least one fluorescent label, characterized in that a fluorescent conjugate comprising an oligonucleotide bonded to a rare-earth metal cryptate is introduced into the measuring medium.

Method for reducing fluorescence quenching in bioassays

The invention relates to a process for reducing the fluorescence quenching caused by the measuring medium in a fluorescence assay, by introducing into said medium of rare earth metal cryptates which are not very sensitive to this fluorescence quenching, said rare earth metal cryptates comprising at least one pyridine radical which is substituted one or more times or unsubstituted. The invention also relates to novel rare earth metal cryptates which are not very sensitive to the fluorescence quenching caused by the measuring medium.

Rare earth metal cryptates which are not very sensitive to the fluorescence quenching

The present invention relates to a method of measuring the luminescence emitted in a luminescent assay, which makes it possible to correct certain perturbations due to the measuring medium. The method comprises employing at least one luminescent tracer compound and a luminescent compound used as an internal reference, which, when exposed to the same excitation wavelength, are capable of emitting at different wavelengths, &#x3bb;and &#x3bb;respectively, either by direct luminescence or by the induction of a luminescent emission, and correcting the measurement of the luminescence emitted by the tracer compound at wavelength &#x3bb;on the basis of the measurement of the luminescence emitted by the reference compound at wavelength &#x3bb;. The invention further relates to the use of the method in a homogeneous method of detecting and/or determining an analyte, and to a device for carrying it out.

Method of measuring the luminescence emitted in a luminescent assay

Phycobiliprotein-linker peptide complex fluorescent tracer and methods

Method of reducing interference in a fluorescent assay

The invention relates to rare earth cryptates consisting of at least one rare earth salt complexed by a macropolycyclic compound corresponding to one of formulae I or II below, in which: the ring ##STR1## is the N.sub.2 O.sub.4 macrocycle, the N.sub.2 O.sub.3 macrocycle or the bis--bipyridine macrocycle; Y is a spacer arm or group which consists of a divalent organic radical selected from linear or branched C.sub.1 to C.sub.20 alkylene groups optionally containing one or more double bonds and/or optionally being interrupted by one or more heteroatoms such as oxygen, nitrogen, sulfur or phosphorus, or from C.sub.5 to C.sub.8 cycloalkylene groups or C.sub.6 to C.sub.14 arylene groups, the said alkylene or arylene groups optionally being substituted by alkyl, aryl or sulfonate groups; Z is a functional group capable of bonding covalently with a biological substance; R is a methyl group or represents the group --Y--Z; and R' is hydrogen or a group --COOR', in which R' is a C.sub.1 to C.sub.10 alkyl group and preferably represents the methyl, ethyl or tert.-butyl group, or alternatively R' is a group --CO--NH--Y--Z. ##STR2##

Rare earth cryptates, processes for their preparation, synthesis

The invention relates to a method of amplifying the emission signal of a luminescent compound in a luminescent assay, and to its use in a luminescent method of detecting and/or determining an analyte in a medium in which it may be present.

Method of amplifying the emission signal of a luminescent compound

Rare earth macrocyclic complexes and use thereof for reduction of

The invention relates to macropolycyclic rare earth complexes, namely cryptates which are useful as fluorescent tracers.

Macropolycyclic rare earth complexes and application as fluorescent

The invention relates to rare earth cryptates consisting of at least one rare earth salt complexed by a macropolycyclic compound corresponding to one of formulae I or II below, in which:

The present invention relates to a homogeneous process for the detection and/or determination of an analyte in a medium in which it may be present, by disclosing the reaction product of the analyte and a corresponding receptor, process consisting in: 1) adding to said medium a first reagent consisting of a receptor for the said analyte; 2) adding a second reagent consisting of at least one of the components of the reaction product of the analyte and at least one of its receptors; one of the two reagents being coupled with a luminescent compound and the other reagent possessing a heavy atom or units containing a heavy atom; 3) incubating the medium after addition of each reagent or after the addition of both reagents; 4) exciting the resulting medium and 5) measuring at equilibrium or during the kinetics, the signal emitted by the luminescent compound, said signal being modulated by the heavy atom effect.

Growing community of inventors

Bagnols sur Ceze, France

Gerard Mathis