Agilent Technologies, Inc.

University of Colorado

Geraldine F Dellinger

Douglas J Dellinger

Joel Myerson

Marvin H Caruthers

Zoltan Timar

Agnieszka Sierzchala

Michel G M Perbost

Zoltan Kupihar

John Turner

Fernando Ferreira

John S Hargreaves

Agnieska Sierzchala

Michel G M Perobost

Agnieszka B Sierzchala

A method of deprotecting a solid support bound polynucleotide comprising at least one 2'-protected ribonucleotide in which a step of contacting the polynucleotide with a composition comprising a diamine is performed under conditions sufficient to deprotect and cleave the polynucleotide which remains retained on the solid support.

Protected monomer and method of final deprotection for RNA synthesis

A method of deprotecting a solid support bound polynucleotide includes the step of contacting the polynucleotide with a composition comprising a diamine under conditions sufficient to deprotect the 2'-protected ribonucleotide residue. The solid support bound polynucleotide has at least one 2&#x2032;-protected ribonucleotide residue, which has the following structure: wherein Bis a protected or unprotected heterocycle; Ris a protecting group selected from a hydrocarbyl, a substituted hydrocarbyl, an aryl, and a substituted aryl; X is O or S; and PG is a thionocarbamate protecting group.

Aspects of the invention include 2' protected nucleoside monomers that are protected at the 2&#x2032; site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.

Thiocarbon-protecting groups for RNA synthesis

Methods of deprotecting polynucleotides are disclosed. One aspect of the method of deprotecting polynucleotides, among others, includes: providing a polynucleotide, wherein the polynucleotide includes at least one nucleotide monomer that has at least one protecting group selected from the following: a base having a protecting group, a 2'-hydroxyl protecting group, and a combination thereof, and deprotecting at least one of the protecting groups of the polynucleotide by introducing the polynucleotide to a solution including an &#x3b1;-effect nucleophile.

Solutions, methods, and processes for deprotection of polynucleotides

Nucleoside monomers, nucleic acids, e.g., oligonucleotides and polynucleotides, methods of making each, methods of deprotecting each, and the like are disclosed herein. Aspects of the invention include 2' silyl containing thiocarbonate protecting groups. Corresponding compositions and methods are provided.

2'-silyl containing thiocarbonate protecting groups for RNA synthesis

In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.

Cleavable thiocarbonate linkers for polynucleotide synthesis

Thiocarbonate linkers for polynucleotides

Nucleotide monomers, polynucleotides, methods of making each, methods of deprotecting each, and the like are disclosed herein.

Monomer compositions for the synthesis of RNA, methods of synthesis, and methods of deprotection

A method for synthesizing a polynucleotide is disclosed. The method comprises providing a functionalized support comprising a triaryl methyl linker bound to the solid support through a substitution on one of the aryl groups, a nucleotide chain bound to the central methyl carbon of the triaryl methyl group and a nucleoside with a reactive site hydroxyl. The method further provides contacting that functionalized support with a precursor having a hydroxyl protecting group and a phosphorous derivative reactive group to produce internucleotide linkage. The resulting compound is then treated to both remove the hydroxyl protecting group on the precursor, and to oxidize the internucleotide bond.

Method of polynucleotide synthesis using modified support

Cleavable linkers for polynucleotides

Methods of forming polynucleotides are disclosed.

Methods and compounds for polynucleotide synthesis

A method of fabricating polynucleotide arrays includes dissolving a nucleotide monomer, oligonucleotide, or polynucleotide in a solvent containing ionic liquid and depositing the resulting solution on an array substrate. The method has particular application to fabrication of an addressable array of polynucleotides on a substrate that carries substrate bound moieties each with a hydroxyl group. The process may be repeated at specific locations on the array to elongate the polynucleotide deposited on the array.

Use of ionic liquids for fabrication of polynucleotide arrays

Methods of forming an internucleotide bond are disclosed. Such methods find use in synthesis of polynucleotides. The method involves contacting a functionalized support with a precursor having an exocyclic amine triaryl methyl protecting group under conditions and for a time sufficient to result in internucleotide bond formation. The functionalized support includes a solid support, a triaryl methyl linker group, and a nucleoside moiety having a reactive site hydroxyl, the nucleoside moiety attached to the solid support via the triaryl methyl linker group. In particular embodiments, the precursor has the structure: wherein:

Growing community of inventors

Boulder, CO, United States of America

Geraldine F Dellinger