The Liposome Company, Inc.

New York University

Syracuse University

Marine Biological Laboratory

Gerald Weissmann

Andrew S Janoff

Alan L Weiner

Marc J Ostro

James R Seibold

Philip B Dunham

An immunoassay utilizing a novel liposome composition in which there is incorporated a stabilizing or destabilizing component and an antigen. The presence of cognate antibodies in test samples is detected by the alteration of the supramolecular structure of the liposomes resulting in changes in stabilization. Destabilization can be detected, and in certain cases, caused by, the addition of magnesium or calcium ions. Increased stabilization can be detected by the polymerization of bilayer components in response to ultraviolet light. Antibody-antigen interactions at the liposome surface mediate the stabilization/destabilization response.

Liposome composition for lupus assay

Disclosed is a method for measuring aggregation in sponge cells using a clinical aggregometer. In addition, using the marine sponge cell as an in vitro model for neutrophil aggregation, a method to determine the relative anti-inflammatory properties of non-steroidal anti-inflammatory pharmaceutical agents by clinical aggregometry is also disclosed.

Method for measuring anti-inflammatory properties of a composition

An assay for detecting SLE antibodies utilizing a novel liposome composition in which there is entrapped a divalent cation responsive indicator. The presence of such antibodies is detected by their stabilization of the supermolecular structure of the liposomes. Such stabilization can be detected, and in certain cases, caused by, the addition of magnesium or calcium ions.

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New York, NY, United States of America

Gerald Weissmann