Alfa Wassermann, Inc.

Ares-serono Research & Development Limited Partnership

Serone Diagnostics Partners

Gerald John Allen

Grenville Arthur Robinson

Gordon C Forrest

Philip Missing

Madlyn D Denyer

An independently rotatably mounted container may be used, by agitating the contents thereof, in the performance of a multi-component reaction or assay, by selective rotation of said container in the presence of adjacently mounted component containers which are not rotated. Such a principle may be applied in suspending solid phases in, for example, automated immunoassay apparatus or in reconstituting freeze dried reagents. Selective rotation has a number of advantages including gentleness to components and greater efficiency and reliability.

Selective component agitation apparatus and method

Method of assay normalized with non-antigen calibrator

The present invention provides a method of performing an immunoassay of a ligand in a liquid sample wherein two independently measureable enzyme labels are separately conjugated to two or more components or populations of components of the assay system and, after completion of the complexing reaction, substantially all of the first enzyme label and a proportion of the second enzyme label are removed from the assay mixture, the proportion of the second label removed being related to the amount of the said ligand and the assay determined from a measurement of said proportion of the second label being normalised by comparison with a measurement of the total first label removed. In this way, manual pipetting errors and other assay procedural errors can be corrected for.

Double-labeled enzymeimmunoassay methods

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Windlesham, United Kingdom

Gerald John Allen