Roche Diagnostics Operations, Inc.

Boehringer Mannheim Gmbh

Microgenics Corporation

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Gerald F Sigler

Mitali Ghoshal

Erasmus Huber

Raymond Hui

Richard Terry Root

Herbert Von Der Eltz

Salvatore Joseph Salamone

Allan R Dorn

Ina Deras

Ronald Charles Hawley

John W Patterson

Eva Hoess

Riaz Rouhani

Alan J McNally

Sigrun Metz

Peter Kern

Christopher Douglas Wilsey

Nigel A Surridge

Todd P Glancy

Stephen S Vitone

Zhi David Deng

Robert J McEnroe

Walter W Jernigan

Rebecca W Muddiman

William A Coty

Frank E Klein

Anlonga Ouyang

Charles F Walter

Michael Joseph Powell

Aniruddha Patwardhan

Lili Arabshahi

Anlong Ouyang

Wei W Yuan

David Davoudzadeh

Jane S C Tsai

Jeffrey E Shindelman

Anthony J Sanchez

Shaker Rashid

Charles E Durant

David Davoudzaheh

Paul R Morrill

Raymond A Hui

Erasmus J Huber

Allan Dorn

Yifei Wu

Activated haptens useful for generating immunogens to HIV protease inhibitors, immunogens useful for producing antibodies to HIV protease inhibitors, and antibodies and labeled conjugates useful in immunoassays for the HIV protease inhibitor saquinavir. The novel haptens feature an activated functionality at the central, non-terminal hydroxyl group. Also described are monoclonal antibodies specific for saquinavir having less than 10% cross-reactivity with lopinavir, nelfinavir, amprenavir, ritonavir, and indinavir, and a murine hybridoma producing said antibodies.

Protease inhibitor conjugates and antibodies useful in immunoassay

Saquinavir conjugates and antibodies useful in immunoassay

A highly specific homogeneous assay method for an immunosuppressive drug using an immunophilin in a single receptor format is provided. In the simplest format, a single receptor is utilized analogous to a competitive immunoassay whereby an immunophilin is substituted for an antibody, and a competition results between a drug conjugate and the drug analyte for a limited number of immunophilin binding sites. In a microparticle agglutination assay format, an immunophilin is either bound to a particle or in solution. In the case where an immunophilin is bound to a particle, a polyvalent conjugate of the drug analyte is present in solution.

Single receptor assays for immunosuppressive drugs

The present invention comprises novel analogs of ecstasy-class compounds and novel ecstasy-class immunogens leashed out of, i.e., derived from, the methylenedioxy position. The invention also comprises unique monoclonal antibodies generated using MDO-leashed MDMA immunogens as well as unique conjugates and tracers. These antibodies, conjugates, and tracers are useful in immunoassays for the detection of ecstasy-class compounds in biological fluids.

Ecstasy-class derivatives, immunogens, and antibodies and their use in detecting ecstasy-class drugs

The present invention provides hapten derivatives useful for the preparation of antigens, antibodies and reagents for use in immunoassays for the detection of LSD and 2-oxo-3-hydroxy LSD. In the present invention, the 2-oxy LSD nucleus is derivatized out of the indole nitrogen to form an aminoalkyl derivative. The resulting haptens can then be further modified at this functionalized position for linking to appropriate immunogenic or labeling groups to provide reagents for immunoassays having substantially equal specificity for both LSD and 2-oxo-3-hydroxy-LSD.

Immunoassay for LSD and 2-oxo-3-hydroxy-LSD

The present invention provides a method for the measurement of an analyte in biological samples whereby an uncompetitive inhibitor is coupled to a ligand and utilized in a homogeneous assay. The analyte can be a drug or drug derivative, hormone, polypeptide, or oligonucleotide. The present invention also provides novel compounds, assay reagents and packaged kits useful for performing such measurements.

Conjugates of mycophenolic acid

Analogs of 6-monoacetyl morphine (6-MAM) are described. These include analogs derivatized at either the C-3 position, the C-6 position, or the nor position of the molecule. These analogs allow for elaboration with linkers terminated by a functional group such as an activated ester, the functional groups being useful for attaching the molecule to other entities such as proteins, polysaccharides, and reporter groups.

6-monoacetylmorphine derivatives useful in immunoassay

The invention provides derivatives of efavirenz and methods of making derivatives of efavirenz. The derivatives include immunogenic compounds for producing antibodies to efavirenz and labeled efavirenz tracers. These compounds are useful in immunoassay methods for determining efavirenz.

Reagents for detecting efavirenz

Derivatives and conjugates of indinavir for generation of antibodies and labeled conjugates for use for detection of indinavir in biological samples. The derivatives are synthesized out of the indane ring hydroxyl group or the pyridine ring nitrogen of indinavir. Also disclosed is synthesis of a major metabolite of indinavir (M6) in a single step from indinavir using palladium catalyst and hydrogen gas. Indinavir M6 has been extended to synthesize various analogs of indinavir with suitable functional groups. These derivatives are useful in the development of indinavir immunogens, antibodies, and labeled conjugates in the development of indinavir immunoassays.

Indinavir derivatives useful in immunoassay

Activated haptens useful for generating immunogens to HIV protease inhibitors, immunogens useful for producing antibodies to HIV protease inhibitors, and antibodies and labeled conjugates useful in immunoassays for HIV protease inhibitors. The novel haptens feature an activated functionality at the central, non-terminal hydroxyl group common to all HIV protease inhibitors, e.g., saquinavir, nelfinavir, indinavir, amprenavir, ritonavir, lopinavir, and atazanavir.

Growing community of inventors

Carmel, IN, United States of America

Gerald F Sigler