Abberior Instruments Gmbh

Max-Planck-Gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Gerald Donnert

Christian Wurm

Stefan W Hell

Lars Kastrup

Benjamin Harke

Roman Schmidt

Matthias Reuss

Andreas Schoenle

Vladimir Belov

Katrin Willig

Kirill Kolmakov

Johanna Wildanger

Shamil Nizamov

Heiko Schill

Christian Eggeling

Joachim Fischer

Matthias Henrich

Winfried Willemer

Andreas Schönle

Joern Heine

Haugen Mittelstaedt

Vladimir N Belov

Haugen Mittelstädt

Jörn Heine

Christian A Wurm

A fluorescence microscope () includes a sample illumination beam path including a source () for illumination light, a first wave front modulator () for providing the focused illumination light () with a central intensity minimum, a beam splitter () and a second adjustable wave front modulator () arranged in a pupil plane () of an objective (). A first detection beam path section including the second wave front modulator () and a telescope () and ending at the beam splitter () coincides with the sample illumination beam path. A separate second detection beam path section includes a detector () for luminescence light from a sample. The telescope () images a first pupil () formed in the pupil plane () in a smaller second pupil (), and transfers a beam of the illumination light () collimated in the second pupil () into an expanded beam collimated in the first pupil ().

Fluorescence microscope with stabilized adjustment and group of components and module for upgrading a fluorescence microscope

In methods of high-resolution imaging a structure of a sample, the structure being marked with fluorescence markers, the sample is subjected to a light intensity distribution including an intensity maximum of focused fluorescence excitation light to selectively scan partial areas of interest of the sample. Fluorescence light emitted out of the sample is registered and allocated to a respective location of the light intensity distribution in the sample. The subjection of the sample to at least one part of the light intensity distribution is terminated at each location of the light intensity distribution, if at least one criterion of the following criteria is met: (a) a predetermined maximum light amount of the fluorescence light emitted out of the sample has been registered, and (b) a predetermined minimum light amount of the fluorescence light emitted out of the sample has not been registered within a predetermined period of time.

Methods of high-resolution imaging a structure of a sample, the structure being marked with fluorescence markers

A scanner head for high-resolution scanning fluorescence microscopy comprises

Scanner head and device with scanner head

A device comprises two polarization-selective optical elements for separately modulating wave fronts of two components of a collimated light beam, which are transversally polarized in orthogonal directions. The two polarization-selective optical elements are first and second partial areas of one spatial light modulator (SLM) diffracting the light beam in backward direction. A mirror arranged between the first and second partial areas of the SLM reflects the light beam coming from the first partial area towards the second partial area. A wave plate arranged between the first partial area and the second partial area of the SLM rotates the polarization directions of both components of the light beam by 90&#xb0;. The mirror reflects the first and second components of the light beam as parallel bundles of light rays resulting in a lateral offset between the first and second components of the light beam behind the second partial area of the SLM.

Device for separately modulating the wave fronts of two components of a light beam and microscope comprising the device

The invention relates to novel fluorescent dyes with phosphorylated hydroxymethyl groups, a method for preparing the same as well as to their use in imaging techniques. The fluorescent dyes are coumarin, rhodamine or BODIPY dyes having of one of the following general formulae I-III: wherein W=OP(O)YYor P(O)YY, where Yand Yindependently denote any of the following residues: OH, O(&#x2212;), ORand OR, NHRand NHR, NRRand NRR, ORand NHR, ORand NRR, NHRand NRR; and any salt thereof.

Fluorescent dyes with phosphorylated hydroxymethyl groups and their use in light microscopy and imaging techniques

The inventive method for optically measuring a sample consists in temporarily repeatedly transmitting an electromagnetic signal () to the sample in such a way that a substance contained in the sample is transferred from a first electronic state () into a second electronic state (), wherein at least one part of said substance in the second state () emits photons which are used for carrying out the optical measurement of the sample, the signal () is transmitted to the same sample area at a certain repetition interval and said repetition interval of the signal () is adjusted with a lifetime of the second state () of the substance having an order of magnitude of 1 ns on a value of at least 0.1 &#x3bc;s which is optimized with respect to photon yield from the substance.

Method and device for optically measuring a sample

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Goettingen, Germany

Gerald Donnert