Promega Corporation

Georgyi V Los

Keith V Wood

Chad Zimprich

Dieter Klaubert

Mark McDougall

Lance P Encell

Monika G Wood

Aldis Darzins

Robert F Bulleit

Tonny Johnson

Chad Zimbrich

Robet F Bulleit

Chard Zimprich

Mark Mcdougall

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

Covalent tethering of functional groups to proteins

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule

Covalent tethering of functional groups to proteins and substrates therefor

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as &#x2022; methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

Compositions comprising a dehalogenase substrate and a contrast agent and methods of use

Compositions comprising a dehalogenase substrate and a radionuclide and methods of use

Polynucleotide encoding a mutant dehalogenase to allow tethering to functional groups and substrates

Compositions comprising a dehalogenase substrate and a fluorescent label and methods of use

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Madison, WI, United States of America

Georgyi V Los