The State University of New York

Georgiy Gudkov

Vera Gorfinkel

Boris Gorbovitski

Dmitri N Gavrilov

Andriy Tsupryk

Dmytro Gudkov

Marina Tcherevishnik

Ivan Tovkach

Dmitriy Gavrilov

A fiberized single photon sensitive spectrometer based on a 32-channel PMT sensor is highly sensitive with broad detection dynamic range. The spectrometer enables accurate and high speed detection, identification and analysis of biological samples labeled with multiple fluorescent markers, such as compositions of multi-color fluorescence signals or radiation emitted by multiple fluorescence dyes. A fiberized optical input of the spectrometer allows an easy and efficient coupling to any measurement system based on fiber collection of the analyzed fluorescence. The spectrometer provides highly accurate DNA sequencing. A 32 channel PMT single photon detector has a detection dynamic range of more than 20 bits and has a frame rate of about 3300 frames per second. The dynamic range of the detector's pixels reaches 10photocounts per second.

Single photon spectrometer

The present invention is directed to a system and method for cross-talk cancellation for multi-lane fluorescence detectors. The invention may be implemented in accordance with a variety of systems, including systems for multi-capillary electrophoresis. The present invention is based on a special calibration procedure for determination of a channel cross-talk matrix and enables an accurate separation of the fluorescence emitted from individual capillary lanes. The proposed method for cross-talk calibration and removal is very useful for design and development of multi-lane single photon counting detection systems.

System and method for cross-talk cancellation in a multilane fluorescence detector

Growing community of inventors

Coram, NY, United States of America

Georgiy Gudkov