Lesaffre Et Compagnie

Université D'auvergne Clermont 1

Université Du Droit Et De La Santé Lille 2

Centre National De La Recherche Scientifique

Institut National De La Recherche Agronomique

Université De Droit Et De La Santé De Lille 2

Georges Pignede

Thomas Desfougeres

Jean-Michel Bavouzet

Jennifer Techel

Didier Colavizza

Maud Petit

Isabelle Mouly

Benoît Thomas

Brieuc Morvan

Christophe Rave

Anaïs Roussel

Alexander Matthias Farwick

Jean-Marc Nicaud

Jean-Charles Bartolucci

Daniel Poulain

Jean-Luc Simon

Pascal Vandekerckove

Arlette Darfeuille-Michaud

Claude Gaillardin

Melanie Legros

Emilie Fritsch

Pierre Desreumaux

Adeline Sivignon

Claire Thorel

Sébastien Hulin

Arlette Darfeuille - Michaud

Strains ofyeast that are genetically modified so as to co-express a gene coding a glucoamylase of fungal origin, a gene coding a glucoamylase ofvar., and a gene coding a xylanase of fungal origin. The production yield of bioethanol through these strains is greater than that of strains that are otherwise identical but that do not include the gene coding the xylanase of fungal origin. Also, a method for obtaining these yeasts, as well as the use of these yeasts in the production of bioethanol.

strains expressing exogenous glucoamylase and xylanase enzymes and their use in the production of bioethanol

The invention relates to a genetically modified fungal microorganism for the production of frambinone, the microorganism having the following characteristics: &#x2014;the capacity to produce frambinone from tyrosine; and &#x2014;a limited capacity or no capacity to break tyrosine down into tyrosol, p-hydroxyphenylacetaldehyde and/or p-hydroxyphenylacetate; and to the use of same for producing frambinone.

Production of frambinone by a recombinant fungal microorganism

The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid, in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form.

Yeast strain having improved capability for fermenting xylose in the presence of acetic acid

The present application relates to improvedyeast strains that co-express a gene encoding a glucoamylase of fungal origin and a gene encoding the glucoamylase ofvar. The present invention also relates to a method for obtaining these yeast strains involving The yeast strains according to the invention are of particular interest in producing bioethanol.

Yeast strains co-expressing exogenous glucoamylases, the method for obtaining said yeast strains and the use thereof to produce bioethanol

The present invention relates to methods for obtaining yeast strains suitable for metabolizing arabinose, and to improved strains with good performance as regards their capacity to ferment arabinose as well as xylose and glucose, including in the presence of inhibitors such as acetic acid.

Obtaining high-performance yeast strains for metabolizing arabinose

The invention concerns the use of the MCM7 gene to confer resistance to an organic acid, preferably acetic acid, to a yeast strain during glucose fermentation.

Use of MCM7 to obtain acetic acid-resistant yeast strains

The present invention relates to a method for obtaining a strain capable of efficiently propagating in a low nutritive potential medium, capable of metabolizing pentoses and of resisting fermentation inhibitors, comprising the following steps; a) growth of a strain of recombinant yeast with a strain of wild yeast lacking any impairments, the recombinant yeast strain comprising at least one copy of an exogenous gene of xylose isomerase and at least one additional copy of a gene of D-xylulokinase included in the genome and linked to a single sexual characteristic of the strain, b) at least two cycles of genome shuffling by sporulation and or random hybridization, c) selection of the population obtained in step b) according to a suitability criterion of the strains to metabolize xylose, d) selection of the population obtained in step c) according to a suitability criterion of the strains to grow in a Pref type medium, a medium with low nutritive value. The invention also relates to a cell, a yeast or a strain of recombinant yeast obtained according to the method, and to the use of such a cell for the production of bioethanol.

Pentose-fermenting strain with optimized propagation

The present invention relates to novel broad spectrum strains of baker's yeast, i.e. yeast strains that are effective on both dough known as unsweetened dough and dough known as sweet dough.

Bread yeast strains

The present invention concerns novelyeast strains capable of multiplying on a substrate comprising at least one C5 sugar with a speed and rate of multiplication compatible with the industrial production of yeast. It also concerns novel strains which, when cultured, make it possible to obtain yeasts having an application efficiency, i.e. an efficiency that is satisfactory in applications and uses of interest in industries such as breadmaking, biomass production, flavour production, the production of secondary metabolites, protein production, ethanol production, brewing, winemaking or the production of yeast extract.

Yeast strains for the production of biomass on a substrate comprising a C5 sugar

The subject matter of the present invention is novel yeast strains capable of metabolizing xylose and resistant to at least one fermentation inhibitor, and also to the method of obtaining same. The subject of the present invention is also the yeasts obtained by culturing said yeast strains and the use thereof for producing at least one fermentation product, preferably ethanol, in particular in a culture medium comprising xylose and at least one fermentation inhibitor.

Yeast strains capable of metabolizing xylose and resistant to inhibitors, method for obtaining same and use thereof

The present invention relates to a method for obtaining improved strains of yeast by integrating at least one gene of interest in a region linked to a sex expression locus, followed by cross-breeding. The present invention also relates to gene integration cassettes and to vectors that can be used in the context of said method. The present invention also relates to improved strains of yeast obtained by the above method, the derived strains of yeast and the yeasts obtained by culturing said strains of yeast.

Method for obtaining improved strains of yeast

The present invention relates to methods for obtaining ethanol-producing yeast strains, yeast strains thus produced, and the industrial production of ethanol from said strains. More particularly, the present invention describes in its most general aspect, a method for preparing yeasts fromstrains by means of the integration, into the genome of the yeast, of at least one gene encoding xylose isomerase and of at least one gene encoding xylitol dehydrogenase. The strains of the invention are useful for producing ethanol from a medium comprising at least one pentose, preferably xylose or a mixture of xylose and arabinose.

Method for preparing an industrial yeast, industrial yeast, and application to the production of ethanol from at least one pentose

The present invention relates to the field of methods for obtaining ethanol-producing yeast strains, to the field of the thus produced strains and to the field of the industrial production of ethanol from said strains. Particularly, the present invention relates, in the most general aspect thereof, to a method for preparing yeasts from industrialstrains, to said strains, and to the use thereof in the industrial production of ethanol from industrial media containing at least one pentose.

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Marcq en Baroeul, France

Georges Pignede