Institut National De La Recherche Agronomique

Georges Pelletier

Michel Renard

Régine Delourme

Catherine Primard-Brisset

Jean-Pierre Poupard

Nicolas Pierre Poupard, Legal Representative

Marion Hélène Poupard, Legal Representative

Raymonde Horvais

Françoise Budar

Jan Drouaud

Agnès Fourgoux

Philippe Guerche

Dominique Lancelin

Francedillaoise Budar

Sandrine Bonhomme

Francoise Budar

Regine Delourme

The invention relates to a method of producing a double low restorer line offor Ogura cytoplasmic male sterility (cms) presenting a radish introgression carrying the Rfo restorer gene deleted of the radish Pgi-2 allele and recombined with the Pgi-2 gene from, and having a good agronomic value characterized by female fertility, a good transmission rate of Rfo and a high vegetative vigour. The invention relates also to a method of forminghybrid seeds and progeny thereof and to the use of markers for selection.

[object Object]

The invention concerns a microspore-specific promoter and its use for obtaining plants with gametophytic male sterility with inducible fertility. It also concerns a method for obtaining hybrid plants.

Microspore-specific promoter and method for obtaining hybrid plants

Ogura sterility DNA is used to develop agronomically valuable hybrids that lack the undesirable features of plants having Ogura DNA (chlorosis at low temperature; poor fertile fertility; aberrant flower morphology) but still impart efficient male sterility (which can be easily restored). Ogura sterility is carried by a DNA sequence defined by nucleotides 928-2273 of a 2428 base sequence reproduced in the specification. When present in mitochondrial or nuclear genome of a plant it confers cytoplasmic male sterility. Also disclosed are recombinant plant nuclear and mitochondrial genomes containing a DNA sequence defined by nucleotides 928-1569 of the specified sequence (or its homologous); cytoplasm containing such a mitochondrial genome; Brassica plants or hybrids containing the sterility DNA; and, DNA probes of at least 10 bases from the 928-1569 sequences. DNA probes are used to detect male sterility and for selecting clones without the need for lengthy agronomic/rehybridization steps.

DNA sequence imparting cytoplasmic male sterility, mitochondrial genome,

Growing community of inventors

Bures sur Yvette, France

Georges Pelletier