Medimmune Limited

The United States of America, as Represented by the Secretary, Department of Health and Human Services

Medimmune Vaccines, Inc.

Other

George William Kemble

Chin-Fen Yang

Gregory Duke

Chongguang Liu

Richard R Spaete

Hong Jin

Brian R Murphy

Erich Hoffmann

Zhongying Chen

Bin Lu

James F Young

Kanta Subbarao

Zhaoti Wang

Richard Schwartz

George Robert Trager

Erich Hoffman

Greg Duke

Bernard Roizman

Vu Truong-Le

Palani Balu

Luisa Yee

Harshvardhan Mehta

Kanta Subbaro

Mark Prichard

Eric Tsao

Chongquang Liu

C G Liu

Brian Murphy

Chiaoyin Kathy Wang

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.

Multi plasmid system for the production of influenza virus

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25&#xb0; C. (and immunogenic compositions comprising the same) is also provided.

Polypeptides, polynucleotides, methods, compositions, and vaccines comprising (avian pandemic) influenza hemagglutinin and neuraminidase variants are provided.

Influenza hemagglutinin and neuraminidase variants

Polypeptides, polynucleotides, methods, compositions, and vaccines comprising influenza hemagglutinin and neuraminidase variants are provided.

The present invention provides novel canine pol I regulatory nucleic acid sequences useful for the expression of nucleic acid sequences in canine cells such as MDCK cells. The invention further provides expression vectors and cells comprising such nucleic acids as well as methods of using such nucleic acids to make influenza viruses, including infectious influenza viruses.

Methods and compositions for expressing negative-sense viral RNA in canine cells

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided.

Methods and compositions for the optimization and production of refrigerator-temperature stable virus, e.g., influenza, compositions are provided. Formulations and immunogenic compositions comprising refrigerator-temperature stable virus compositions are provided.

Refrigerator-temperature stable influenza vaccine compositions

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Sunnyvale, CA, United States of America

George William Kemble