Harvard College

Other

Massachusetts Institute of Technology

University of California

Gen9, Inc.

The General Hospital Corporation

University of Washington

The Brigham and Women's Hospital, Inc.

Government of the United States of America, as Represented by the Secretary of Commerce

Roche Sequencing Solutions, Inc.

Genomatica, Inc.

Reg Life Sciences, LLC

Joule Unlimited Technologies, Inc.

Good Start Genetics, Inc.

Ls9, Inc.

George M Church

Luhan Yang

Prashant G Mali

Evan R Daugharthy

Richard C Terry

Morten Sommer

Je-Hyuk Lee

Gautam Dantas

Jeremy Agresti

Amy Rowat

Daniel Levner

Michael Super

Jin-Woong Kim

Liang-Yin Chu

David A Weitz

Gregory J Porreca

Francois Vigneault

Jehyuk Lee

Marc Guell

Mark Umbarger

Kevin Michael Esvelt

Kun Zhang

Srivatsan Raman

Noah D Taylor

Frederic Vigneault

Marc J Lajoie

Chao-ting Wu

Alejandro Chavez

Frank J Poelwijk

Joseph M Chou

Christopher John Gregg

Benjamin W Pruitt

Henry Hung-yi Lee

Reza Kalhor

Hon Man Alex Ng

Edward Stuart Boyden

Joseph M Jacobson

Fei Chen

Daniel Branton

David W Deamer

Larry Li-Yang Chu

Sriram Kosuri

Daniel J Mandell

Shahar Alon

Jonathan Scheiman

Jameson K Rogers

Joyce Lichi Yang

Charles Towne

Parastoo Khoshakhlagh

Feng Zhang

David Arthur Berry

Jay D Keasling

Andreas W Schirmer

Zhihao Hu

Mark Albert Akeson

Stephen B Del Cardayre

Le Cong

Adam H Marblestone

Amy J Wagers

Jay Ashok Shendure

Stephen G Kieffer-Higgins

Shane A Brubaker

Ido Bachelet

Robi David Mitra

John J Kasianowicz

Paul Warren Tillberg

Son C Nguyen

Lisa C Friedman

Mohammadsharif Tabebordbar

Farren J Isaacs

Kettner John Frederick Griswold, Jr

Mirkó Palla

Liangcai Gu

Uri Laserson

Ying Kai Chan

Jeffrey Matthew Nivala

Chris Somerville

Robert Samuel Langer

Jun Zhu

David Baker

Carlo Giovanni Traverso

Jennifer Ann Lewis

Frank Anthony Skraly

Ove Öhman

Aruna Ayer

Asmamaw T Wassie

Brian D Green

David B Kolesky

Nikolai A Eroshenko

Mark Andrew Skylar-Scott

Anna I Mandinova

Kimberly A Homan

Kalim U Mir

Johan Elf

Christine Elaine Tinberg

Shawn M Douglas

Nili Ostrov

Carl Magnus Schoellhammer

Jeffrey D Macklis

The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subject to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.

Assays and other reactions involving droplets

Methods of editing target nucleic acids are provided using a guide RNA and a Cas9 protein to excise exons in a target gene and where the edited gene is expressed to produce a truncated polypeptide.

RNA-guided systems for in vivo gene editing

The present disclosure is directed to compositions and methods for deriving a plurality of kinetics parameters () for at least two different enzyme variants in a multiplex manner using nanopore-based sequencing. In some embodiments, the systems and methods may be used to screen different nanopore variants, or different combinations of both nanopore variants and enzyme variants.

Enzyme screening methods

The technology described herein provides viral capsid polypeptides bearing mutations that alters tissue tropism of a virus comprising the viral capsid polypeptide. In various embodiments, tissue tropisim to the heart, kidney, liver, lung, spleen, or blood is altered.

Viral vectors exhibiting improved gene delivery properties

The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer.

Methods of labelling one or more subcellular components (e.g., an organelle and/or subcellular region) in vivo are provided. Methods of labelling a protein in vivo are provided. Methods of determining a nucleic acid sequence in situ are also provided.

Methods for high-throughput labelling and detection of biological features in situ using microscopy

The present disclosure provides methods of improving nuclease-mediated gene targeting frequencies in cells using PFTα or bFGF.

Methods for increasing efficiency of gene editing in cells

Methods for attaching barcodes to polypeptides are provided. Methods for detecting molecular interactions at the single molecule level are provided. Embodiments of the invention are directed to a ONA barcoded protein array technology for parallel protein interaction profiling on a single molecule basis. DNA barcodes are attached to proteins collectively via ribosome display or individually via enzymatic conjugation. Novel methods are described herein that measure protein interactions based on the statistical analysis of co-localized polonies arising from barcoding DNAs of interacting proteins.

Barcoded protein array for multiplex single-molecule interaction profiling

Methods of gene therapy are provided for treating or preventing age-related diseases or conditions by regulating one or more functional proteins associated with age-related diseases or conditions.

Gene therapy methods for age-related diseases and conditions

Methods of selectively sequencing amplicons in a biological sample are provided.

Method of selectively sequencing amplicons in a biological sample

Provided herein are pluripotent stem cells comprising particular combinations of transcription factor for the production of oligodendrocyte progenitor cells (OPCs). Also provided herein are methods of producing the pluripotent stem cells and methods of using the pluripotent stem cells to produce (OPCs) and oligodendrocytes.

Methods and compositions for the production of oligodendrocyte progenitor cells

Compositions and methods for making a plurality of probes for analyzing a plurality of nucleic acid samples are provided. Compositions and methods for analyzing a plurality of nucleic acid samples to obtain sequence information in each nucleic acid sample are also provided.

Methods for making nucleotide probes for sequencing and synthesis

A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.

Multiplexed genome editing

The invention generally relates to methods of performing a capture reaction. In certain embodiments, the method involves obtaining a nucleic acid, fragmenting the nucleic acid, and capturing a target sequence on the nucleic acid fragment using a capture moiety, such as a molecular inversion probe.

Capture reactions

The present invention relates to methods of synthesizing de novo a polynucleotide storing data using a programmable template independent polymerase.

Methods of storing information using nucleic acids

Growing community of inventors

Brookline, MA, United States of America

George M Church