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The United States of America As Represented by the United States

George L Gaines

J Mark Weber

David C Demirjian

Malcolm J Casadaban

Douglas G Johnson

Michael P O'Neil

Michael R Wasielewski

Mark P Niemczyk

David J Gosztola

The instant disclosure teaches transposons and methods of the instant invention for making Protein Fusions by rapid, random shuffling of protein domains to produce novel protein fusions. This system is generally applicable to production of multifunctional chimeric proteins in vivo and in vitro. The methods and constructs of the instant invention can be used to randomly create both carboxy- and amino-terminal protein fusions in vivo. The methods and contructs of the instant invention are useful in the development of a protein domain library, in the construction of multifunctional enzymes, and in the accelerated evolution of new enzymatic activities.

Protein fusion method and constructs

A light-intensity dependent molecular switch comprised of a compound which shuttles an electron or a plurality of electrons from a plurality of electron donors to an electron acceptor upon being stimulated with light of predetermined wavelengths, said donors selected from porphyrins and other compounds, and a method for making said compound.

Organic solid state switches incorporating porphyrin compounds and

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River Forest, IL, United States of America

George L Gaines