Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

George John Carman

J William Maney

Geoffrey A Barrall

Jason Komadina

Charlotte Yang

Takeshi Harada

J William Maney Jr

Geoffrey Barrall

Systems and methods for inserting a single pore into a membrane are described herein. A stepped or ramped voltage waveform can be applied across the membranes of the cells of an array, where the voltage waveform starts at first voltage and increases in magnitude over a period of time to a second voltage. The first voltage is selected to be low enough to reduce the risk of damaging the membrane, while the rate of voltage increase is selected to provide sufficient time for the pores to insert into the membranes. Once a pore is inserted into the membrane, the voltage across the membrane rapidly drops, thereby reducing the risk of damaging the membrane even if the applied voltage between the electrodes is further increased.

Systems and methods for self-limiting protein pore insertion in a membrane

Growing community of inventors

San Mateo, CA, United States of America

George John Carman