Pioneer Hi-bred International, Inc.

Other

E.i. Dupont De Nemours and Company

The University of Arizona

George J Hoerster

William James Gordon-Kamm

Keith S Lowe

Carolyn A Gregory

Theodore Mitchell Klein

Ronald D Burnett

Rebecca E Cahoon

Kevin E McBride

Brian A Larkins

Brian R Dilkes

Yumin Tao

Matthew A Bailey

Sonriza Rasco-Gaunt

Young Min Woo

Xifan Sun

Ning Wang

John A McElver

Alexandre Da Silva Conceição

Shiv Bahadur Tiwari

Xinli Emily Wu

Ramgopal Nadimpalli

Carlos M La Rota

Maren L Arling

Dennis L Bidney

Craig E Hastings

Margit C Ross

Sheila E Maddock

Ajith Anand

Todd J Jones

Laura A Church

Patrea M Hill

Carolyn Ann Gregory

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Methods and compositions for rapid plant transformation

The invention provides isolated polynucleotides, specifically Cyclin D polynucleotides, and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants for the purpose of increasing transformation efficiency.

Cyclin D polynucleotides, polypeptides and uses thereof

The invention provides novel methods of using viral replicase polypeptides and polynucleotides. Included are methods for increasing transformation frequencies, increasing crop yield, providing a positive growth advantage, modulating cell division, transiently modulating cell division, and for providing a means of positive selection.

Methods of using viral replicase polynucleotides and polypeptides

The invention provides methods of transforming plants and plant cells including recalcitrant cells with isolated nucleic acids encoding LEC1 transcriptional activators to induce transformation or regeneration efficiency, apomixis or somatic embryogenesis.

'Methods of using leci transcriptional activator nucleic acids'

The invention provides isolated nucleic acids and their encoded proteins which act as transcriptional activators and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts.

Isolated LEC1 transcriptional regulator encoding nucleic acids and methods of use thereof

The invention provides isolated polynucleotides and their encoded proteins which act as transcriptional activators and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts.

LEC1 trancriptional activator nucleic acids and methods of use thereof

The invention provides novel methods of using viral replicase polypeptides and polynucleotides. Included are methods for modulating endoreduplication and increasing crop yield.

The invention provides isolated polynucleotides and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants.

The invention provides improved plant transformation methods. In particular the method provides increased transformation freequency, especially in recalcitrant plants. The method comprises stably transforming a target cell with at least one polynucleotide of interest. The target cell has been previously transformed to stimulate growth of the cell and has gone through at least one cell division.

Methods for enhancing plant transformation frequencies

Growing community of inventors

Des Moines, IA, United States of America

George J Hoerster