Takara Bio USA, Inc.

Clontech Laboratories, Inc.

George G Jokhadze

Alex Chenchik

Craig Betts

Nathalie Bolduc

Sayantan Mitra

Steve Oh

Robert Bibilashvilli

Grigoriy Simeonov Tchaga

Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.

Spin columns comprising poly(acid) membrane separation matrices, and methods of making and using the same

Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3' hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.

Methods for adding adapters to nucleic acids and compositions for practicing the same

The present invention provides metal ion affinity peptides, fusion proteins comprising metal ion affinity peptides, and polynucleotides encoding the fusion proteins. A feature of the subject invention is that the metal ion affinity peptide has a formula selected from the group consisting of: formula 1: (His-X-X)-(His-X-X-X)-(His-X), wherein each of Xand Xis independently an amino acid with an aliphatic or an amide side chain, each of X, X, Xis independently an amino acid with a basic side chain (except His) or an acidic side chain, each Xis an amino acid with an aliphatic or an amide side chain, n1 and n2 are each independently 1&#x2013;3, and n3 is 1&#x2013;5; formula 2: (His-Asn), where n=3 to 10; and formula 3: (His-X-X), wherein each of Xand Xis an amino acid having an acidic side chain, and n=3 to 10. The invention further provides recombinant vectors comprising subject polynucleotides, and host cells comprising the recombinant vectors. The invention further provides methods and kits for purifying a fusion protein comprising a metal ion affinity peptide.

Polynucleotides encoding metal ion affinity peptides and related products

Methods and compositions are provided for analyzing differences in the RNA profiles between a plurality of different physiological samples. In the subject methods, a set of a representational number of distinct gene specific primers is used to generate labeled nucleic acids from each of the different physiological samples. The labeled nucleic acids are then compared to each other and differences in the RNA profiles are determined. The subject methods find use in methods of identifying differential gene expression.

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Mountain View, CA, United States of America

George G Jokhadze