E.i. Dupont De Nemours and Company

Other

George F Dunn, Jr

Joseph D Irr

John D Walker

Viet X Nguyen

Lise N Halpern

This invention relates to containers for the cryopreservation of mammalian cells and particularly for the long-term freezing of red blood cell. This invention also relates to methods of manufacturing such containers.

Blood cryopreservation container

A lymphocyte-containing white blood cell fraction obtained by standard leukapheresis, elutriation leukapheresis or standard centrifugation can be used for production of lymphokine activated killer cells by incubation with IL-2. Removal of red blood cells and granulocytes by centrifugation on ficoll is not necessary.

Simplified method for the preparation of human lymphokine activated

A process for separating particles from mixtures containing the particles is disclosed. An adoptive transfer therapy process wherein mononuclear cells are separated from a mixture of blood cells by the process is also disclosed. The process comprises supporting the mixture in a centrifuge chamber; subjecting the chamber to a centrifugal force; and forcing a displacing fluid through the chamber, against the centrifugal force, thereby separating the preselected particles from the mixture. The process is further characterized in that the displacing fluid has a density greater than or equal to the density of the preselected particles.

Growing community of inventors

Wenonah, NJ, United States of America

George F Dunn, Jr