Xy, Inc.

Colorado State University Research Foundation

Other

Cytomation, Inc.

George E Seidel

John Louis Schenk

Tae Kwang Suh

Lisa Ann Herickhoff

Kehuan Lu

David G Cran

Juan F Moreno

Kristopher Scott Buchanan

Maurice A Rosenstein

Douglas H Ferguson

Matthias J G Ottenberg

George C Malachowski

Patrick M McCue

Edward L Squires

Kristopher S Buchanan

John L Xy Inc Schenk

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw () and an incubation element () that can be sealed with a cap ().

Method of in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

System and method for sorting cells

A sperm cell process system providing to generate sperm cell insemination samples having controlled sperm cell fertility characteristics of sperm cells.

Sperm cell separation system

Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformily stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.

Methods for separating frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations

In-vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations

A sex selected equine embryo production system for the production of sex selected equine embryos and offspring by fertilization of oocytes with sex selected sperm by intracytoplasmic sperm injection.

Sex selected equine embryo production system

A heterogeneous inseminate including a first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species for the production of sex-selected embryos and sex-selected offspring.

Heterogeneous inseminate system

Low pressure sperm cell separation system

Non-surgical artificial insemination is achieved for sexed and unsexed equines in a commercially practical manner and with dosages of insemination sperm which were not previously thought to be practical for commercial implementation. Practical and field usable techniques for insemination are presented as well as techniques which offer success ratios at level comparable to the existing routine, high dosage unsexed artificial insemination techniques in equines. Improved insemination and sorting systems particularly adapted to use for sex-selected sperm are disclosed.

Method of establishing an equine artificial insemination sample

Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformly stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.

Separation systems of frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations

Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa () that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformity stain () spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.

System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

Multiple sexed embryo production system for bovine mammals

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a HEPES bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

Growing community of inventors

Laporte, CO, United States of America

George E Seidel