Cornell University

Regents of the University of Minnesota

Louisiana State University and Agricultural & Mechanical College

Other

George Barany

Robert P Hammer

Francis Barany

Maria Kempe

Matthew Lubin

Monib Zirvi

Herman Blok

Fernando Albericio

Phillip Belgrader

Samuel Zalipsky

Jane Chang

Knud J Jensen

Micheal F Songster

Jordi Alsina

Norman P Gerry

Joseph M Day

Nuria A Sole

Nuria A Sol E

Karin Musier-Forsyth

Qinghong Xu

Lin Chen

Josef Vagner

Josef V&aacute;gner

Nancy E Witowski

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays

The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence difference.

Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions

Detection of nucleic acid sequence differences using coupled polymerase chain reactions

The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

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Falcon Heights, MN, United States of America

George Barany