Gsf-forschungszentrum Fur Umwelt Und Gesundheit, Gmbh

Gsf-forschungszentrum Fuer Umwelt Und Gesundheit Gmbh

Georg W Bornkamm

Martin Staege

Axel Polack

Bettina Kempkes

Wolfgang Hammerschmidt

Dirk Eick

Lothar J Strobl

Ursula Zimber-Strobl

Nicola Maria Jochner

T cells having a desired antigen specificity are stimulated by (a) introducing immortalizing genes into antigen-presenting cells in a manner permitting regulation of the expression and/or function of at least one of these genes to achieve conditionally immortalized antigen-presenting cells; (b) introducing a gene encoding the desired antigen into the immortalized cells in a manner permitting the antigen to be expressed after the expression and/or abolishment of the function of at least one of the immortalizing genes stops; (c) expanding the immortalized antigen-presenting cells by expression and/or functional activation of the immortalizing genes; (d) completing the proliferation of the immortalized antigen-presenting cells by stopping the expression and/or abolishing the function of at least one of the controllable immortalizing genes; (e) continuing the expression of the antigen; (f) adding leucocytic cells including T cells and cultivating the cell mixture to stimulate the T cells directed against the desired antigen; and (g) optionally purifying and isolating the stimulated T cells.

Method for the stimulation of T cells having a desired antigen specificity

The invention is directed to a method for the preparation of a conditionally immortalized immortalization-helper cell (fuseme), the fusemes generated by said method, hybridoma cells prepared using said fusemes as well as a method for immortalization of mammalian cells using said fuseme cells. Further, the invention relates to the generation of T cells directed agaist tumor cells using a fuseme cell.

Method for the immortalization of cells using conditionally transformed helper cells

The present invention provides a process for the preparation of human monoclonal antibodies from human B cells. According to this process, human antibody-producing B cells are immortalized by infection with an Epstein-Barr virus, or derivative thereof, that expresses Epstein-Barr virus nuclear antigen 2 (EBNA2). EBNA2 function, which is required for immortalization, is subsequently shut off, which results in an increase in antibody production. The invention provides a variety of systems that allow expression of EBNA2 function to achieve immortalization, followed by reduction of EBNA2 expression or function to facilitate antibody production.

Process for the preparation of human monoclonal antibodies and their use

DNA construct and in vitro test for detecting tumor promoters by means

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Munich, Germany

Georg W Bornkamm