Zymogenetics, Inc.

Alderbio Holdings LLC

Alder Biopharmaceuticals, Inc.

Bristol-myers Squibb Compnay

Geoffrey F Lee

John A Latham

Leon F Garcia-Martinez

Ethan Wayne Ojala

Daniel S Allison

Mark Young

Bruce L Zamost

Leon F Garcia-martinez

Steven D Davin

Hoa Binh Do

Robert M Dedinsky

Processes for producing and purifying recombinant proteins are disclosed. In particular, the present disclosure provides processes of producing and purifying multi-subunit proteins expressed in yeast or filamentous fungal cells. The production and/or purification of such proteins are monitored for impurities, preferably using lectin binding assays, such that one or more process parameters may be adjusted to maximize the amount of desired recombinant protein and minimize the amount of glycosylated impurities. The processes can also be monitored for other undesired product-associated impurities, such as aggregates and nucleic acids. In exemplary embodiments, the recombinant proteins are multi-subunit proteins, such as antibodies, the host cell is a yeast, such as, and the glycosylated impurity is a glycovariant of the desired recombinant polypeptide, such as an N-linked and/or O-linked glycovariant.

Antibody purification and purity monitoring

The expression vectors and methods using anexpression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.

Production and purification of IL-29

Growing community of inventors

Mercer Island, WA, United States of America

Geoffrey F Lee