Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Iprasense Sas

Horiba Abx Sas

Glaxosmithkline Intellectual Property Development Limited

Ipratech Sa

Geoffrey Esteban

Cedric Allier

Lionel Herve

Thomas Bordy

Cédric Allier

Pierre Blandin

Damien Isebe

David Sergeant

Thierry Poskin

Anais Ali Cherif

Jérémie Cubeta

Martin Pisaneschi

Melissa Hill

A method for determining a state of a cell, the cell being placed in a sample in contact with a culture medium, includes illuminating the sample with a light source and acquiring an image of the sample with an image sensor, the image sensor lying in a detection plane. From the acquired image, a position of the cell in a plane parallel to the detection plane is located and a refractive index or a relative refractive index of the cell is estimated, the relative refractive index corresponding to a refractive index of the cell relative to the refractive index of the culture medium. From the estimation, an index of interest of the cell is determined. From the index, a state of the cell among predetermined states is classified, the predetermined states including at least one apoptosis state and one living state.

Method for determining the viability of cells

An analysis system features a confinement device, an optical measurement device, a flow device, a collecting conduit, and an actuation device. The confinement device comprises a measurement chamber, optically transparent first and second measurement surfaces that face each other across a distance that defines a thickness of the measurement chamber, and a flexible membrane that forms a seal with the measurement surfaces to laterally delimit the measurement chamber. When the collecting is sealed with a container, a connection is established that permits a liquid sample to be collected from the container. The optical measurement device emits light towards the chamber and detects light that has been transmitted through it, wherein the flow device causes liquid to flow through the collecting conduit between the container and the measurement chamber. The actuation device varies the thickness.

Analysis system including a confinement device with variable-size measurement chamber partially delimited by a flexible membrane

A method for holographic characterization of a particle contained in a sample, based on an image, or hologram, of the sample obtained by an image sensor when the sample is illuminated by a light source. The hologram is the subject of a holographic reconstruction, to obtain a reference complex image, representative of the light wave transmitted by the sample in a reconstruction plane. A holographic propagation operator is applied to the reference complex image, to obtain a plurality of secondary complex images, from which a profile is determined describing the change in an optical feature of the light wave transmuted by the sample along the axis of propagation of the light wave.

Holographic method for characterizing a particle in a sample

A method for identifying a state of a cell contained in a sample, including: illuminating the sample using a light source by producing an incident light wave propagating toward the sample; then acquiring, using a matrix-array photodetector, an image of the sample, the sample being placed between the light source and the matrix-array photodetector such that the matrix-array photodetector is exposed to a light wave resulting from interference between the incident light wave and a diffraction wave produced by each cell; applying a numerical reconstruction algorithm to the image acquired by the matrix-array photodetector, to estimate a characteristic quantity of the light wave reaching the matrix-array detector, at a plurality of distances from the matrix-array photodetector. The value of the characteristic quantity, or its variation as a function of distance, allows the state of the cell to be determined from among predetermined states.

Method for determining the state of a cell

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Teyran, France

Geoffrey Esteban