Pacific Biosciences of California, Inc.

Foundation Medicine, Inc.

Genentech, Inc.

Geoffrey Alan Otto

Stephen Wilson Turner

Doron Lipson

Roman Yelensky

Philip James Stephens

Sean R Downing

Alexander Nevin Parker

Mikhail G Shapiro

John S Eid

Paul S Peluso

Devon Murphy

Mima Jarosz

Jonas Korlach

Daniel Bernardo Roitman

Gene W Shen

Mirna Jarosz

Robin A Emig

Mathieu Foquet

Thang Pham

David R Rank

Xiangxu Kong

David Kagen Hanzel

John Lyle

Jie He

David Fabrizio

David Shames

Mark Kennedy

Michelle Nahas

Travis Clark

Shan Zhong

Daniel Lieber

Craig Anthony Cummings

Yan Li

Sarah Margaret Shagan

Erica Beth Schleifman

David Hanzel

Alexander N Parker

A method of analyzing a tumor sample comprising:

Optimization of multigene analysis of tumor samples

Methods of evaluating or providing a clonal profile of a subject interval, e.g., a subgenomic interval, or an expressed subgenomic interval (or of a cell containing the same), in a subject, are disclosed.

Multigene analysis of tumor samples

The present invention provides therapeutic, diagnostic, and prognostic methods for cancer. The invention provides methods of treating a cancer, methods of determining whether an individual having a cancer is likely to respond to a treatment including an immune checkpoint inhibitor (e.g., a PD-L1 axis binding antagonist), methods of predicting responsiveness of an individual having a cancer to a treatment including an immune checkpoint inhibitor (e.g., a PD-L1 axis binding antagonist), methods of selecting a therapy for an individual having a cancer, methods of providing a prognosis for an individual having a cancer, and methods of monitoring a response of an individual having a cancer, based on a blood tumor mutational burden (bTMB) score or a maximum somatic allele frequency (MSAF) from a sample (e.g., a whole blood sample, a plasma sample, a serum sample, or a combination thereof) from the individual.

Therapeutic and diagnostic methods for cancer

The present invention provides methods and compositions for performing illuminated reactions, particularly sequencing reactions, while mitigating and/or preventing photodamage to reactants that can result from prolonged illumination. In particular, the invention provides methods and compositions for incorporating photoprotective agents into conjugates comprising reporter molecules and nucleoside polyphosphates.

CIS reactive oxygen quenchers integrated into linkers

Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.

Active surface coupled polymerases

The invention provides methods and compositions for the controlled termination of polymerase mediated primer extension reactions. The methods and compositions of the invention are broadly useful, and in a preferred aspect can be used in identifying sequence elements of template nucleic acids. Control of termination not only provides temporal control over termination, but, when used in conjunction with optically confined reaction regions, also spatially controls such termination.

Controllable strand scission of mini circle DNA

Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.

Mitigation of photodamage in analytical reactions

Surface comprising DNA polymerase having mutations bound through an affinity tag

System for the mitigation of photodamage in analytical reactions

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Brookline, MA, United States of America

Geoffrey Alan Otto