Bio-rad Laboratories, Inc.

Geoff McDermott

Benjamin J Hindson

Christopher Hindson

Amy L Hiddessen

Dianna Maar

John Frederick Regan

Chunxiao Han

Erin Rae Chia

Claudia Litterst

Adam Lowe

Svilen Tzonev

Yann Jouvenot

Yaqi Wang

Ben Hindson

Erin Chia

Svilen S Tzonev

Chris Hindson

Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

Compositions, methods and systems for polymerase chain reaction assays

Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.

Multiplexed digital assay with specific and generic reporters

The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

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Pleasanton, CA, United States of America

Geoff McDermott