Tsinghua University

Capitalbio Corporation

Gengxin Lan

Dong Wang

Jing Cheng

Huafang Gao

Li Li

Fan Yang

Rui Zhang

Chi Zhang

Qian Chen

Yuxiang Zhou

Bin Cai

Tao Guo

Yue Tian

Yiming Zhou

Yanhua Liu

Yizhe Zhang

Ze Li

Yangzhou Jiang

Chuanzan Zhao

Xuemei Ma

The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.

Hybridization methods using natural base mismatches

This invention relates generally to the field of nucleic acid analysis. In particular, the invention provides a method for typing a target gene, using, inter alia, a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon an oligonucleotide probe complementary to said target nucleotide sequence and at least one of the following oligonucleotide control probes: a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe. Oligonucleotide probes or probes arrays for typing a HLA target gene are also provided.

DNA chip based genetic typing

Growing community of inventors

Beijing, China

Gengxin Lan