Shenzhen Mindray Bio-Medical Electronics Co., Ltd.

Gengwen Chen

Ziqian Zhang

Zhaoyang Li

Yi Ye

Bo Liu

Jian Xie

Ruixia Song

Provided are a method for preparing reticulocyte simulating particles and platelet simulating particles, and a reference control. The method for preparing the reticulocyte simulating particles comprises: staining mammalian anucleated red blood cells having a volume of 60-120 fL with a protein fluorescent dye activated by N-hydroxysuccinimide, and fixing the anucleated red blood cells to prepare the reticulocyte simulating particles. The platelet simulating particles are prepared from mammalian anucleated red blood cells having a volume of 2-25 fL, and the steps of preparing the platelet simulating particles are the same as that for the reticulocyte simulating particles. The preparation method comprises: using an protein fluorescent dye activated by N-hydroxysuccinimide to stain mammalian anucleated red blood cells having different volumes, so as to respectively obtain reticulocyte simulating particles and platelet simulating particles. The fluorescence and volume direction thereof in the scatter diagram are similar to the scatter diagram distribution of reticulocytes, reticulated platelets and platelets in fresh blood. The prepared simulating particles have good stability and do not interfere with the counting and differentiation of other cell particles.

Method for preparing reticulocyte simulating particles and platelet simulating particles, and reference control

A blood detection method is provided, including: treating a blood sample with a first reagent to obtain a test sample, wherein the first reagent includes a hemolytic agent for lysing red blood cells in the blood sample into fragments having light scattering characteristics significantly different from those of platelets; passing particles in the test sample through a detection area of an optical detection system one by one, to obtain optical information of the test sample; and obtaining optical information of platelets according to at least two types of the optical information of the test sample. In the method, a platelet count is obtained by lysing red blood cells in a blood sample, and white blood cell subpopulations can be also differentiated.

Blood detection method and blood analysis system

A reagent, a method for differentiating platelets under a hemolysis condition using the reagent and a blood cell analyzer are provided. The reagent includes a first reagent as a hemolytic agent, and a second reagent which is a membrane-specific dye or a mitochondrion-specific dye. By using the reagent, the method and the blood cell analyzer, platelets can be differentiated and an alarm about reticulocytes can be provided under a hemolysis condition, and white blood cell can further be classified and counted, thereby quickly and accurately analyzing a blood sample in a single channel.

Reagent, method for analyzing platelets and blood cell analyzer

The disclosure provides a sample testing method and a sample analyzer, the method including: providing a sample and a reagent, the reagent including at least two fluorescent dyes for staining particles in the sample, wherein an absolute value of a difference between wavelengths corresponding to peaks of emission spectra of the two fluorescent dyes is greater than 30 nanometers and less than 80 nanometers, and an overlap between the emission spectra of the two fluorescent dyes is not greater than 50%; mixing the sample and the reagent to form a sample solution; flowing the sample solution in a flow cell in a single test, irradiating the particles flowing in the flow cell using light with a single wavelength; detecting at least fluorescence signals generated by the particles; and obtaining a test result of the sample based on at least the fluorescence signals.

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Shenzhen, China

Gengwen Chen