Bridgestone Corporation

Gen Suzuki

Hiroshi Mouri

Akio Kusano

Masato Hiruma

A method of making a cell sheet comprising secondary spheroids, including (a) obtaining cardiosphere-derived cells; (b) cultivating the cardiosphere-derived cells for a first period of time in a first media comprising at least one of an ascorbic acid and an analog thereof, to form secondary spheroids; (c) transferring an amount of the spheroids formed in step (b) into a mold; (d) culturing the secondary spheroids for a second period of time in a second media comprising at least one of the ascorbic acid and an analog thereof, wherein the at least one of the ascorbic acid and an analog thereof is present in an amount effective to promote a formation of an extracellular matrix; and (e) culturing a product obtained in step (d) for a third period of time, in the absence of the at least one of the ascorbic acid and an analog thereof.

Cardiosphere-derived cell sheet and methods of making and using the same

A pneumatic tire comprising a tread portion which comprises a surface rubber layer which is disposed in an outer portion of the tread portion in the radial direction of said tire and has a specific resistance of 10 &OHgr;&middot;cm or more after curing by vulcanization and conductive rubber members which comprise a rubber composition having a specific resistance of 10 &OHgr;&middot;cm or less and replace a portion of the surface rubber layer, wherein the conductive rubber members are disposed in portions of the surface rubber layer extending from the outer surface to the inner surface in the radial direction of said tire discontinuously in the circumferential direction of said tire at the outer surface of the surface rubber layer in the radial direction of said tire, is provided. The pneumatic tire shows not only an excellent effect of static discharge but also excellent durability.

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Tokyo, Japan

Gen Suzuki