Ajinomoto Co., Ltd.

Nara Institute of Science and Technology

National University Corporation Nara Institute of Science and Technology

Gen Nonaka

Kazuhiro Takumi

Osamu Kurahashi

Kazuhiko Matsui

Tsuyoshi Nakamatsu

Shinichi Sugimoto

Yoshio Kawahara

Eiichiro Kimura

Seiko Hirano

Shunsuke Yamazaki

Tsuyoshi Osumi

Naoki Akiyoshi

Yumi Matsuzaki

Kanae Nakamura

Hiroshi Takagi

Natalia Viktorovna Stoynova

Ekaterina Alekseevna Savrasova

Iwao Ohtsu

Yoshihiko Hara

Rie Takikawa

Takeshi Nakatani

Natthawut Wiriyathanawudhiwong

A bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an amino acid such as L-cysteine and has been modified to have specific mutation in the yeas gene, is cultured in a medium, and the L-amino acid is collected from the medium.

L-amino acid-producing bacterium and a method for producing an L-amino acid

A method for producing L-cysteine and the like is provided by developing a novel technique for improving bacterial L-cysteine-producing ability. The method includes the steps of culturing a bacterium belonging to the genuswhich has L-cysteine-producing ability, and in which expression of a gene involved in sulfite reduction is enhanced, in a medium containing thiosulfate, and collecting L-cysteine, a related substance thereof, or a mixture thereof which accumulate in the medium.

Method for producing L-cysteine

A novel technique for improving the production by bacteria of amino acids that contain sulfur has been developed, and thereby a sulfur-containing amino acid-producing bacterium, and a method for producing a compound such as a sulfur-containing amino acid are provided. A sulfur-containing amino acid, a related substance thereof, or a mixture of these can be produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has a sulfur-containing amino acid-producing ability and has been modified so that the activity of the protein encoded by the yeeE gene, for example, the protein of the following (A) or (B), is increased in a medium, and collecting a sulfur-containing amino acid, a related substance thereof, or a mixture of them from the medium: (A) a protein having the amino acid sequence of SEQ ID NO: 14, (B) a protein having the amino acid sequence of SEQ ID NO: 14 but which includes one or several amino acid substitutions, deletions, insertions, or additions, and when the intracellular activity of this protein is increased, the ability of the bacterium to produce a sulfur-containing amino acid is improved.

Sulfur-containing amino acid-producing bacterium and method for producing sulfur-containing amino acid

A method for producing an L-amino acid is described using a bacterium of the Enterobacteriaceae family, wherein the bacterium contains a protein which is able to confer resistance to growth inhibition by L-cysteine.

Method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family

An L-cysteine-producing bacterium is provided, as well as a method for producing L-cysteine etc. using the bacterium by developing a novel technique for improving L-cysteine-producing ability of a bacterium. By culturing a bacterium belonging to the family Enterobacteriaceae, which has L-cysteine-producing ability and is modified so that activity of a protein encoded by the yciW gene, for example, a protein defined by the following (A) or (B), is reduced, in a medium, and collecting L-cysteine, L-cystine, a derivative thereof, or a mixture thereof from the medium, these compounds are produced: (A) a protein having the amino acid sequence shown in SEQ ID NO: 2, (B) a protein having the amino acid sequence shown in SEQ ID NO: 2, but which includes substitution, deletion, insertion, or addition of one or several amino acid residues, reduction of which activity in the bacterium results in improvement in the L-cysteine-producing ability.

L-cysteine-producing bacterium and a method for producing L-cysteine

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The present invention describes a bacterium belonging to the familywhich has L-cysteine-producing ability and has been modified to decrease the activity of a protein encoded by the d0191 gene. This bacterium is cultured in a medium, and L-cysteine, L-cystine, derivatives thereof, or a mixture thereof is collected from the medium.

L-cysteine producing bacterium and a method for producing L-cysteine

The present invention provides a bacterium belonging to the family Enterobacteriaceae, which is able to produce L-cysteine and has been modified to increase the activity of the protein encoded by the dsbA gene. This bacterium is cultured in a medium, and L-cysteine, L-cystine, a derivative or precursor thereof or a mixture thereof can be collected from the medium.

L-Cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has L-cysteine-producing ability and has been modified so that the activity of a protein encoded by a tolC gene, for example, a protein defined in the following (a) or (b), is increased in a medium, and by collecting L-cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof from the medium:

An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has an L-amino acid-producing ability and inherently has a native activity of a glucose dehydrogenase that uses pyrroloquinoline quinone as a coenzyme, but has been modified so that the activity of the glucose dehydrogenase is reduced, in a medium, and collecting the L-amino acid from the medium.

L-amino acid-producing microorganism and a method for producing an L-amino acid

The present invention provides a bacterium belonging to the family Enterobacteriaceae which has L-cysteine-producing ability and has been modified to decrease the activity of the protein encoded by the yhaM gene. This bacterium is cultured in a medium, and L-cysteine, L-cystine, their derivatives, or a mixture thereof is collected from the medium.

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from, preferably an enzyme that functions at a higher temperature compared with that of. PCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of

Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived frompreferably an enzyme that functions at a higher temperature compared with that ofPCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of

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Kawasaki, Japan

Gen Nonaka