Seagate Technology Incorporated

Gemma Mendonsa

Tim Rausch

Riyan Alex Mendonsa

Walter Richard Eppler

Krishnan Subramanian

Edward Charles Gage

Martin Giles Blaber

Vivek Krishnamurthy

Eric K Wadleigh

Methods of writing data to a DNA strand by inserting data-encoding oligos or symbols into a DNA backbone. One particular method of synthesizing a DNA strand encoding data includes cleaving a DNA backbone into multiple segments, and pasting a plurality of data-encoding oligo symbols between the multiple segments, with the terminal ends of the segments joining homologous terminal ends of the symbols, resulting in the DNA strand encoding data comprising alternating segments and symbols.

DNA backbone editing for DNA data storage

A microfluidic lab-on-a-chip system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The lab-on-a-chip has a fluidic platform with a plurality of arrays operably connected to a voltage source and a controller for the voltage source, a set of first inlets operably connected to the fluidic platform, each first inlet for one DNA symbol from a DNA symbol library, a set of second inlets operably connected to the fluidic platform, each second inlet for one DNA linker from a DNA linker library, and a mixing area operably connected to the fluidic platform and to the plurality of first inlets and the plurality of second inlets.

Microfluidic lab-on-a-chip for gene synthesis

A Surface-Enhanced Raman Spectroscopy (SERS) device to perform accurate label-free long-read DNA sequencing. A Raman sensor has a hot spot defined by plasmonic nanostructures and excited by at least one laser. An immobilized DNA polymerase can be used to pull a DNA template strand to be sequenced through the hot spot.

Sequencing nucleic acids via surface enhanced Raman spectroscopy

Systems and methods for identifying DNA strand size and purifying the DNA based on strand size using electrophoresis. The methods include moving, via voltage, a plurality of DNA strands through a separation gel from an inlet of a capillary or passage to either a first outlet or a second outlet dependent on the DNA strand length. In some implementations, the system is a capillary electrophoresis system. In other implementations, the system is a microfluidic lab-on-a-chip.

Gel electrophoresis for DNA purification

A microfluidic device for polymerase chain reaction (PCR) processing includes a platform with a microstructure with at least one reaction chamber, and a heating element. The heating element heats at least a part of the at least one reaction chamber. The heating element includes at least one spin torque oscillator (STO) configured to heat the at least a part of the at least one reaction chamber to one or more temperatures for PCR processing.

Microwave heating device for lab on a chip

A system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The symbol library has a number of DNA symbols each having a first overhanging end and a second overhanging end different than and non-complimentary to the first end, the first and second ends being the same nucleotides for each DNA symbol. The linker library has pairs of DNA linkers, a first linker of a pair having a first end and a second end and a second linker of the pair having a first end and a second end, the first end of the first linker being the same nucleotides for each first linker and the second end of the second linker being the same nucleotides for each second linker, wherein the second end of the first linker and the first end of the second linker have complementary nucleotides. The first linker joins to the first end of a DNA symbol and the second linker joins to the second end of another DNA symbol.

Methods of gene assembly and their use in DNA data storage

Method of utilizing a nanochannel in combination with at least one magnetic sensor for detecting (e.g., identifying) molecules, cells, and other analytes. Particularly, the method includes bringing molecules, labeled with magnetic nanoparticles (MNPs), in close proximity to the magnetic sensor to identify the molecules via an output signal from the magnetic sensor. The method is particularly suited for identifying nucleotides of DNA and RNA strands.

Nanochannel with magnetic sensor for the detection of molecules

A system for DNA gene assembly that utilizes a DNA symbol library and a DNA linker library. The symbol library has a number of DNA symbols each having a first overhanging end and a second overhanging end different than and non-complimentary to the first end, the first and second ends being the same nucleotides for each DNA symbol. The linker library has pairs of DNA linkers, a first linker of a pair having a first overhanging end and a second overhanging end and a second linker of the pair having a first overhanging end and a second overhanging end, the first end of the first linker being the same nucleotides for each first linker and the second end of the second linker being the same nucleotides for each second linker, wherein the second end of the first linker and the first end of the second linker have complementary nucleotides. The first linker joins to the first end of a DNA symbol and the second linker joins to the second end of another DNA symbol.

Growing community of inventors

Minneapolis, MN, United States of America

Gemma Mendonsa