Devgen Nv

Monsanto Technology LLC

Geert Plaetinck

Pascale Feldmann

Gregory R Heck

Ty T Vaughn

Claire A CaJacob

Wendy T Maddelein

Irene Nooren

James Arthur Baum

Thierry Bogaert

Romaan Raemaekers

Irene Maria Antonia Nooren

Els Van Bleu

Nicole Damme

Thierry Andre Olivier Eddy Bogaert

Frederic Pecqueur

Lies Degrave

Laurent Kubler

Christ Platteeuw

Isabel Remory

Richard Zwaal

Els Vanbleu

Jean-Pierre Renard

Pascale Feldman

Marc Van De Craen

Marc Georges Logghe

James A Buam

Katharine Mortier

Katherine Mortier

Thierry Bogart

Frédéric Pecqueur

Isabelle Vercauteren

Thierry Andre Bogaert

The present invention relates to control of pest infestation by inhibiting one or more biological functions. The invention provides methods and compositions for such control, By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pest, a reduction in pest infestation is obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

Methods for genetic control of insect infestations in plants and compositions thereof

The present invention relates to control of pest infestation by inhibiting one or more biological functions. The invention provides methods and compositions for such control. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pest, a reduction in pest infestation is obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

The present invention relates to methods for controlling pest infestation using double stranded RNA molecules. The invention provides methods for producing transgenic cells expressing the double stranded RNA molecules, as well as compositions and commodity products containing or treated with such molecules.

Methods for controlling pests using RNAi

The present invention relates to methods for controlling pest infestation using double stranded RNA molecules. The invention provides methods for making transgenic plants that express the double stranded RNA molecules, as well as pesticidal agents and commodity products produced by the inventive plants.

Transgenic plant-based methods for plant pests using RNAI

The present invention relates to methods for controlling pest infestation using double stranded RNA molecules. The invention provides methods for making transgenic plants that express the double stranded RNA molecules, as well as pesticidal agents and commodity products produced by the inventive plants. The invention also provides transgenic plants that are resistant to insect pest infestation.

Transgenic plant-based methods for plant pests using RNAi

Methods for genetic control of insect infestation in plants and compositions thereof

There is provided a method of identifying DNA responsible for conferring a particular phenotype in a cell which method comprises a) constructing a cDNA or genomic library of the DNA of the cell in a suitable vector in an orientation relative to a promoter(s) capable of initiating transcription of the cDNA or DNA to double stranded (ds) RNA upon binding of an appropriate transcription factor to the promoter(s), b) introducing the library into one or more of the cells comprising the transcription factor, and c) identifying and isolating a particular phenotype of the cell comprising the library and identifying the DNA or cDNA fragment from the library responsible for conferring the phenotype. Using this technique it is also possible to assign function to a known DNA sequence by a) identifying a homologue(s) of the DNA sequence in a cell, b) isolating the relevant DNA homologue(s) or a fragment thereof from the cell, c) cloning the homologue or fragment thereof into an appropriate vector in an orientation relative to a suitable promoter(s) capable of initiating transcription of dsRNA from the DNA homologue or fragment upon binding of an appropriate transcription factor to the promoter(s) and d) introducing the vector into the cell from step a) comprising the transcription factor.

Characterisation of gene function using double stranded RNA inhibition

Improved vector constructs useful in the expression of double-stranded RNA are provided. The constructs are particularly useful for expression of double-stranded RNA in vitro and in vivo.

Vector constructs

There is provided a method of identifying DNA responsible for conferring a particular phenotype in a cell which method comprises a) constructing a cDNA or genomic library of the DNA of the cell in a suitable vector in an orientation relative to a promoter(s) capable of initiating transcription of the cDNA or DNA to double stranded (ds) RNA upon binding of an appropriate transcription factor to the promoter(s), b) introducing said library into one or more of the cells comprising the transcription factor, and c) identifying and isolating a particular phenotype of the cell comprising the library and identifying the DNA or cDNA fragment from the library responsible for conferring the phenotype. Using this technique it is also possible to assign a function to a known DNA sequence by a) identifying a homologue(s) of the DNA sequence in a cell, b) isolating the relevant DNA homologues(s) or a fragment thereof from the cell, c) cloning the homologue or fragment thereof into an appropriate vector in an orientation relative to a suitable promoter(s) capable of initiating transcription of dsRNA from the DNA homologue or fragment upon binding of an appropriate transcription factor to the promoter(s), and d) introducing the vector into the cell from step a) comprising the transcription factor.

Characterization of gene function using double stranded RNA inhibition

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Merelbeke-Bottelare, Belgium

Geert Plaetinck