Siemens Healthcare Diagnostics Gmbh

Bayer Corporation

Gary W Rheinheimer

Chris Thomas Zimmerle

Meitak Teresa Yip

Linda Anderson-Mauser

James Arthur Profitt

David A Brock

Jim Mattler

Methods of minimizing hook effect interference in an immunoassay are disclosed. Also disclosed are reagents, kits, and immunoassay devices that may be utilized in accordance with the method.

Devices and methods for minimizing hook effect interference in immunoassays

Provided herein are color charts and water color pigment solutions useful, for example, in the calibration of reflectance-based diagnostic analyzers.

Water color pigment solutions for use with reflectance-based diagnostic analyzers

Disclosed herein are methods of detecting ascorbic acid in a urine sample from a subject, including contacting at least a portion of the urine sample with a test strip including a reagent pad and detecting whether ascorbic acid is present in the urine sample by measuring an intensity of color on the reagent pad, wherein a reduction in the intensity of color on the reagent pad indicates the presence of ascorbic acid.

Detection of ascorbic acid in a urine sample

The inventive concepts disclosed herein are generally directed to the use of a hand-held mobile computing device, such a smartphone or tablet, to interpret the results of a medical assay device. By controlling conditions like image placement and illumination and correcting for imperfect illumination, accurate results can be obtained with hand-held mobile computing device.

Optical interpretation of assay results

Methods and systems are disclosed including a reagent analyzer, comprising a test analyzing mechanism, such as an optical imaging system, configured to read a first sample of a specimen combined with a reagent configured to react with the sample in a presence of an analyte of interest and a second sample of the specimen that is not combined with a reagent, and to output one or more first signals indicative of the test analyzing mechanism reading the first and second samples; and a processor receiving the one or more first signals and executing logic to analyze the second sample responsive to the processor determining that the analyte of interest is present in the first sample. The processor may execute logic to analyze the second specimen utilizing one or more ratio algorithm and comparing the results of the algorithm against predetermined values indicative of expected color.

Reduction of false positive on reagent test devices

An improved agglutination immunoassay is characterized by reacting a sample fluid which may contain an analyte with a generic antibody conjugated to latex particles and then adding an antibody specific to the analyte to be determined. Agglutination resulting from adding the antibody specific to the analyte is measured and correlated with the amount of analyte in the sample.

Generic method for latex agglutination assays

Disclosed is an improvement in the analysis for an analyte in a urine test sample in which the urine is contacted with a labeled antibody specific to the analyte and the concentration of the analyte is determined by measuring the response from the label. The improvement involves maintaining the concentration of urea in the test sample above the threshold value which value is the concentration of urea at which increases in the urea concentration do not affect the accuracy of the assay such as by interfering with the binding between the analyte and the labeled antibody.

Formulation for reducing urea effect for immunochromatography assays using urine samples

Disclosed is an improved method for the detection of an analyte in a fluid test sample using a strip of a negatively charged matrix material having a zone containing mobile, labeled binding partner for the analyte and a separate zone for capturing the labeled binding partner as it is carried through this zone by the fluid test sample. The improvement involves combining the fluid test sample with a polyalkoxylated amine surfactant to control non-specific binding of the labeled binding partner to the negatively charged matrix material.

Growing community of inventors

Goshen, IN, United States of America

Gary W Rheinheimer