University of Massachusetts

St. Jude Children's Research Hospital

Gary S Stein

Andre J Van Wijnen

Janet L Stein

Jane B Lian

Jeanne B Lawrence

Sheldon Penman

Cong-Mei Zeng

Amjad Javed

Scott Hiebert

Daniel W Young

Shari Meyers

Sayyed K Zaidi

Lindsay Shopland

The present invention provides a method useful for analysis of punctate distribution of supramolecular complexes that are compartmentalized in nuclear microenvironments. In preferred embodiments the method provides quantitative analysis of the assembly of regulatory machinery within the context of nuclear architecture and to provide a mechanistic link with biological control. In other embodiments, the invention provides a method of identifying a molecule by parameters of its subnuclear distribution, comprising the steps of quantifying organizational parameters of domains within subnuclear distributions of a molecule; performing factor analysis on the quantified organizational parameters; performing discriminant analysis on the quantified organizational parameters; comparing the results of the factor analysis and the results of the discriminant analysis to one or more standards, thereby identifying a molecule by parameters of its subnuclear distribution.

Quantitative assessment of biological function based on the temporal and spatial organization of subnuclear domains

Nuclear matrix targeting peptides (NMTPs) capable of delivering a variety of therapeutic and diagnostic agents, such as transcription regulatory factors, to the nuclear matrix of a cell are disclosed.

Isolated nuclear matrix targeting peptide

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Shrewsbury, MA, United States of America

Gary S Stein