Diamond Scientific Company

Advanced Genetics Research Institute

University of California

Gary P. Wiesehahn

Laurence Corash

Gary P Wiesehahn

David R Stevens

Richard E Giles

Richard P Creagan

Biological compositions are freed of functional polynucletides by treatment of the biological composition with psoralen derivatives under irradiation conditions in which the proteins retain their original physiological activities and any polynucleotide present is rendered inactive. More specifically blood components are decontaminated of viruses by the addition of psoralen and irradiation and thereafter adding glucose.

Method of blood component decontamination by glucose addition

Vaccines employing inactivated viruses having improved retention of antigenic characteristics are prepared by psoralen-inactivation of the live virus in a non-oxidizing atmosphere. By excluding oxygen and other oxidizing species from the inactivation medium, degradation of the antigen characteristics resulting from irradiation with ultraviolet light is largely prevented. The resulting inactivated viruses are employed in vaccine preparations for the inoculation of susceptible hosts to inhibit viral infection.

Preparation of inactivated viral vaccines

Novel vaccines for feline viral rhinotracheitis are prepared by psoralen inactivation of live Feline Herpesvirus I by exposure to ultraviolet radiation in the presence of an inactivating furocoumarin. The resulting inactivated viruses are suitable as the immunogenic substances in vaccines, which vaccines are useful for inoculation of hosts susceptible to feline virus rhinotracheitis.

FVR vaccine

Biological compositions are freed of functional polynucleotides by treatment of the biological composition with psoralen derivatives under irradiation conditions in which the proteins retain their original physiological activities and any polynucleotide present is rendered inactive.

Photochemical decontamination treatment of whole blood or blood

Biological compositions are decontaminated by treatment with furocoumarin derivatives and irradiation under particular conditions in which the proteins retain their original physiological activities and any pathogenic microorganisms and polynucleotide fragments thereof are rendered inactive. It has been found that reduction of the amount of dissolved oxygen in the treatment solution substantially inhibits denaturation of the proteins.

Novel vaccines for vesicular stomatitis virus are prepared by psoralen inactivation of the live virus under mild conditions in an inert atmosphere. The resulting inactivated virus can be used as a vaccine for inoculation of susceptible hosts to inhibit VSV infection.

Vaccine for the prevention of vesicular stomatitis virus infection

Novel vaccines of double-stranded RNA viruses are prepared by psoralen inactivation under mild conditions in an inert atmosphere, optionally in the presence of a mild singlet oxygen scavenger. The resulting inactivated virus can be used as a vaccine for inoculation of hosts to provide for the stimulation of the immune system to the virus.

Growing community of inventors

Alameda, CA, United States of America

Gary P Wiesehahn