Coulter Corporation

Gary P Toedter

David E Hofheinz

Kenneth H Kortright

Lori A Charie

Samuel R Pearlman

Carole Sullivan

A hybrid cell line is provided which is capable of producing monoclonal antibodies which binds to HTLV-I and HTLV-II core antigens p24 and p53; but does not bind the p19 core antigen. The antibody also binds to core antigens of simian T-cell leukemia virus Type I (STLV-I). The monoclonal antibody is identified as the KC-88 monoclonal antibody. The cell line which produces the KC-88 monoclonal antibody has been deposited in the American Type Culture Collection, Rockville, Md. and assigned A.T.C.C. Deposit No. HB 10562.

Monoclonal antibody for detecting HTLV-I, HTLV-II and STLV-I viruses

A hybrid cell line is provided which is capable of producing monoclonal antibodies which bind to HIV core antigen P55, the precursor protein coded for the gag gene, the core protein P24 and partial breakdown products P39 and P33. The monoclonal antibody embodying the invention does not bind the P18 core protein or any HIV envelope antigens. The cell line of the invention was developed by a unique immunization protocol in which BALB/c mice were immunized over a series of multiple infusions using a select group of immunogens and a conventional myeloma cell line for fusion with the murine splenocytes harvested. The monoclonal antibody is identified as the KC-57 antibody. This monoclonal antibody is especially useful for a solid phase immunoassay in which the monoclonal antibody is detected in its binding to HIV antigens found in a serum or plasma sample from a human patient. The cell line which produces the KC-57 monoclonal antibody has been deposited in the American Type Culture Collection, Rockville, Md. and assigned A.T.C.C. No. HB 9585.

Monoclonal antibody specific to HIV antigens

A hybrid cell line capable of producing monoclonal antibodies uniquely specific to human neutrophils and eosinophils and exhibiting no specificity for lymphocytes, basophils and monocytes. Further there is noreactivity with acute leukemia cells. One of the partners in the hybridoma fusion of a mouse spleen cell developed from using human granulocytes as the immunization agent. The monoclonal antibody further is capable of being used to enumerate and isolate neutrophils in normal peripheral blood and possibly in blood of patients with acute leukemia.

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Miramar, FL, United States of America

Gary P Toedter