Miles Inc.

Miles Laboratories, Inc.

Riebel, Alexander

Oosta, Gary

Himmelmann, Wolfgang

Sobel, Johannes

Schranz, Karl

White, William

Gary Oosta

Alexander Riebel

Wolfgang Himmelmann

Karl Schranz

Helmut Reiff

Gunter Helling

Karl-Wilhelm Schranz

Johannes Sobel

Karl W Schranz

Peter Doppelfeld

William White

Process for the preparation of stable dispersions of hydrophobic reagents in aqueous material, which process involves dissolving an ionomeric polymer, together with hydrophobic reagent substance, in a suitable organic solvent, adding water to the resulting material and then removing the organic solvent. The resulting dispersion has exceptionally high stability and finds utility in the production of diagnostic reagent test devices.

Process for the preparation of reagent layers containing hydrophobic

Proteins such as enzymes are attached directly to a polyamide or cellulose hydrate carrier. Attachment can be carried out by impregnation of the carrier with a protein solution. The carrier can be in the form of a membrane, and the polyamide can be nylon. Resulting carrier-bound proteins find utility as biocatalyst, as test strips or as chromatography materials. In a preferred embodiment, a polyamide or cellulose hydrate membrane carrier is impregnated with an enzyme selected from peroxidase, glucose oxidase, diaphorase and glucose dehydrogenase and then dried, coated with a luminol solution and again dried. The resultant membrane can be used for glucose determination by measuring chemiluminescence produced when the membrane contacts a glucose solution.

Enzymes immobilized on polyamides or cellulose hydrate

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Elkhart, IN, United States of America

Gary Oosta